GNB3 Polyklonaler Antikörper

GNB3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15388-1-AP

Synonyme

GNB3, Transducin beta chain 3

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Galerie der Validierungsdaten

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  WB analysis using 15388-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15388-1-AP (GNB3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15388-1-AP (GNB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15388-1-AP (GNB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15388-1-AP (GNB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15388-1-AP (GNB3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 15388-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15388-1-AP (GNB3 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, HepG2-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Eierstockgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15388-1-AP bindet in WB, IHC, ELISA GNB3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNB3 fusion protein Ag7050
• Vollständiger Name: guanine nucleotide binding protein (G protein), beta polypeptide 3
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 35-37 kDa
• GenBank-Zugangsnummer: BC002454
• Gene symbol: GNB3
• Gene ID (NCBI): 2784
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.