GNB3 Monoklonaler Antikörper

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• KD/KO Validated

GNB3 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2B6E1

Kat-Nr. : 67497-1-Ig

Synonyme

2B6E1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3

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  WB analysis using 67497-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67497-1-Ig

  WB result of GNB3 antibody (67497-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNB3 transfected HepG2 cells.

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  WB analysis using 67497-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 67497-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67497-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat retina using 67497-1-Ig

  rat retina tissue were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67497-1-Ig (GNB3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67497-1-Ig (GNB3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67497-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GNB3 antibody (67497-1-Ig, Clone: 2B6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: hTERT-RPE1-Zellen, Daudi-Zellen, HepG2-Zellen, L02-Zellen, Maushirngewebe, Hausschwein-Hirngewebe, Raji-Zellen, Ramos-Zellen, Rattenhirngewebe, Ratten-Retina-Gewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67497-1-Ig bindet in WB, IHC, IF/ICC, ELISA GNB3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GNB3 fusion protein Ag7050
• Vollständiger Name: guanine nucleotide binding protein (G protein), beta polypeptide 3
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 35-37 kDa
• GenBank-Zugangsnummer: BC002454
• Gene symbol: GNB3
• Gene ID (NCBI): 2784
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.