GNB3 Rekombinanter Antikörper

GNB3 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240281D1

Kat-Nr. : 83452-4-RR

Synonyme

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, 240281D1

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  WB analysis using 83452-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83452-4-RR (GNB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 83452-4-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GNB3 Recombinant antibody (83452-4-RR, Clone:240281D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HeLa using 83452-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GNB3 Recombinant antibody (83452-4-RR, Clone:240281D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83452-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83452-4-RR against Human GNB3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, C6-Zellen, HepG2-Zellen, L02-Zellen, Maushirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83452-4-RR bindet in WB, FC (Intra), ELISA GNB3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GNB3 fusion protein Ag0127
• Vollständiger Name: guanine nucleotide binding protein (G protein), beta polypeptide 3
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 35-37 kDa
• GenBank-Zugangsnummer: BC000115
• Gene symbol: GNB3
• Gene ID (NCBI): 2784
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.