GNL1 Polyklonaler Antikörper

GNL1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 14078-1-AP

Synonyme

HSR1, Guanine nucleotide-binding protein-like 1, GTP-binding protein HSR1

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  WB analysis using 14078-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 14078-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14078-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14078-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14078-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 14078-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 14078-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 14078-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 14078-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 14078-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GNL1 antibody (14078-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 14078-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GNL1 antibody 14078-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, Raji-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

14078-1-AP bindet in WB, IHC, IF/ICC, ELISA GNL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNL1 fusion protein Ag5203
• Vollständiger Name: guanine nucleotide binding protein-like 1
• Berechnetes Molekulargewicht: 607 aa, 69 kDa
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC018366
• Gene symbol: GNL1
• Gene ID (NCBI): 2794
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Guanine nucleotide-binding protein-like 1 (GNL1), also known as HSR1, belongs to HSR1_MMR1 subfamily of nucleolar GTPases. It encodes 607 amino acids with a molecular mass of 69 kDa and contains basic amino acids rich N-terminus, acidic amino acids rich C-terminus and proline rich-domains. GNL1 plays a critical role in liver cell proliferation, but its function remains largely unknown (PMID: 18255255).

Publikationen

Species	Application	Title
human	WB	Front Cell Dev Biol; A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients.; Authors - Zewei Tu