GNPDA2 Polyklonaler Antikörper

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• KD/KO Validated

GNPDA2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 17105-1-AP

Synonyme

GlcN6P deaminase 2, GNP2, GNPDA 2, GNPDA2, SB52

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  WB analysis of rat kidney using 17105-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17105-1-AP (GNPDA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17105-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17105-1-AP (GNPDA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 17105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17105-1-AP (GNPDA2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 17105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17105-1-AP (GNPDA2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 17105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17105-1-AP (GNPDA2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse kidney using 17105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17105-1-AP (GNPDA2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 17105-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17105-1-AP (GNPDA2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 17105-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17105-1-AP (GNPDA2 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattennierengewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Milzgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

17105-1-AP bindet in WB, IHC, ELISA GNPDA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNPDA2 fusion protein Ag10780
• Vollständiger Name: glucosamine-6-phosphate deaminase 2
• Berechnetes Molekulargewicht: 275 aa, 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC015532
• Gene symbol: GNPDA2
• Gene ID (NCBI): 132789
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GNPDA2(Glucosamine-6-phosphate deaminase 2) is also named as GNP2 and belongs to the glucosamine/galactosamine-6-phosphate isomerase family. It catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. Human GNPDA1 and GNPDA2 shared the same fold and were predicted to form hexameric particles(PMID:12965206). This protein has 5 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 29 kDa, 23 kDa and 27 kDa.

Publikationen

Species	Application	Title
human	WB	Glycobiology; Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.; Authors - Sanna Oikari ; KD Validated
mouse	WB	Int J Endocrinol; GNPDA2 Gene Affects Adipogenesis and Alters the Transcriptome Profile of Human Adipose-Derived Mesenchymal Stem Cells.; Authors - Lijun Wu