GOLGA3 Polyklonaler Antikörper

GOLGA3 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Rezensionen (1)

Kat-Nr. : 21193-1-AP

Synonyme

GCP170, Golgi complex-associated protein of 170 kDa, Golgin 160, Golgin-160, MEA 2

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  WB analysis using 21193-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21193-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 21193-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 21193-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GOLGA3 antibody (21193-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Hodengewebe, L02-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IF: See 2 publications below

Produktinformation

21193-1-AP bindet in WB, IF/ICC, ELISA GOLGA3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GOLGA3 fusion protein Ag15531
• Vollständiger Name: golgi autoantigen, golgin subfamily a, 3
• Berechnetes Molekulargewicht: 1498 aa, 167 kDa
• Beobachtetes Molekulargewicht: 167 kDa
• GenBank-Zugangsnummer: BC142658
• Gene symbol: GOLGA3
• Gene ID (NCBI): 2802
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Aβ-induced Golgi fragmentation in Alzheimer's disease enhances Aβ production.; Authors - Gunjan Joshi
human	WB	PLoS Biol; A  conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation.; Authors - Weichao Zhang
		J Cell Sci; SIRT2 deacetylates GRASP55 to facilitate post-mitotic Golgi assembly.; Authors - Xiaoyan Zhang
human	WB	J Biol Chem; GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin.; Authors - Erpan Ahat
human	WB	Mol Biol Cell; Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.; Authors - Stephen C Ireland
mouse,human	WB,IF	PeerJ; Mutation of S461, in the GOLGA3 phosphorylation site, does not affect mouse spermatogenesis; Authors - Changtong Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (04-02-2023)

Very good for WB.

• Applications: Western Blot