GP73/GOLPH2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GP73/GOLPH2 Polyklonal Antikörper für FC, IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 15126-1-AP

Synonyme

golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2, golgi protein 73kD, GOLM1, GOLPH2, GP73, GP73/GOLPH2

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Galerie der Validierungsdaten

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  WB analysis using 15126-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15126-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15126-1-AP

  IP Result of anti-GP73/GOLPH2 (IP:15126-1-AP, 4ug; Detection:15126-1-AP 1:1000) with HeLa cells lysate 2800ug.

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  IHC staining of human liver cancer using 15126-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15126-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15126-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15126-1-AP (GP73/GOLPH2 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton and nuclei were stained with CL594-phalloidin and DAPI, respectively.

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  IF Staining of HeLa using 15126-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (15126-1-AP) at dilution of 1:1800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 15126-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (15126-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 15126-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15126-1-AP (GP73/GOLPH2 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staning with CoraLite555-Phalloidin.

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  FC experiment of HeLa using 15126-1-AP

  1X10^6 HeLa cells were stained with 0.2ug GP73/GOLPH2 antibody (15126-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HepG2-Zellen, HuH-7-Zellen, MDA-MB-231-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Produktinformation

15126-1-AP bindet in WB, IP, IHC, IF, FC, ELISA GP73/GOLPH2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GP73/GOLPH2 fusion protein Ag7207
• Vollständiger Name: golgi membrane protein 1
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC001740
• Gene symbol: GOLM1
• Gene ID (NCBI): 51280
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.

Publikationen

Species	Application	Title
human	WB	PLoS Pathog; GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation.; Authors - Xuewu Zhang ; KD Validated
human	WB	J Cell Sci; Rapid affinity purification of intracellular organelles using a twin strep tag.; Authors - Jian Xiong
human	IF	Chemistry; Investigation of Glycosylphosphatidylinositol (GPI)-Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction; Authors - Sayan Kundu
human	WB,IHC,IF	J Cancer; GP73 level determines chemotherapeutic resistance in human hepatocellular carcinoma cells.; Authors - Jia-Zhou Ye ; KD Validated
		Curr Alzheimer Res; RCAN1 Increases Aβ Generation by Promoting N-Glycosylation Via Oligosaccharyl Transferase.; Authors - T Wang
human	WB	Eur Rev Med Pharmacol Sci; LncRNA MALAT1 knockdown inhibits cell migration and invasion by suppressing autophagy through miR-384/GOLM1 axis in glioma.; Authors - R Ma