Syntabulin Polyklonaler Antikörper

Syntabulin Polyklonal Antikörper für WB, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 16972-1-AP

Synonyme

Syntaxin-1-binding protein, SYBU, KIAA1472, GOLSYN, Golgi-localized syntaphilin-related protein

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  WB analysis of HeLa using 16972-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16972-1-AP (Syntabulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16972-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16972-1-AP (Syntabulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of rat brain using 16972-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 16972-1-AP (Syntabulin antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat brain using 16972-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 16972-1-AP (Syntabulin antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 16972-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16972-1-AP (Syntabulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF-P: Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

16972-1-AP bindet in WB, IF/ICC, IF-P, ELISA Syntabulin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Syntabulin fusion protein Ag9344
• Vollständiger Name: Golgi-localized protein
• Berechnetes Molekulargewicht: 662 aa, 72 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC012082
• Gene symbol: GOLSYN
• Gene ID (NCBI): 55638
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Hum Mol Genet; Loss of FEZ1, a gene deleted in Jacobsen syndrome, causes locomotion defects and early mortality by impairing motor neuron development.; Authors - Saravanan Gunaseelan
mouse,human	WB,IF	Cell Death Discov; Syntabulin regulates neuronal excitation/inhibition balance and epileptic seizures by transporting syntaxin 1B; Authors - Pingyang Ke
mouse	WB	Tissue Cell; Targeting Siglec-15 mediates mitochondrial retrograde regulation of cervical cancer development; Authors - Jing Wang
mouse	WB	Neurobiol Dis; Amyloid beta-mediated KIF5A deficiency disrupts anterograde axonal mitochondrial movement.; Authors - Qi Wang