GOT2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GOT2 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

29

Kat-Nr. : 14800-1-AP

Synonyme

FABPpm, GOT2, KAT4, KATIV, mAspAT, mitAAT, Transaminase A

---

Galerie der Validierungsdaten

• 

  WB analysis using 14800-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 14800-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14800-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14800-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14800-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 14800-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14800-1-AP (GOT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 14800-1-AP (GOT2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14800-1-AP (GOT2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14800-1-AP (GOT2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 14800-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 14800-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GOT2 antibody 14800-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HEK-293 using 14800-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug GOT2 antibody (14800-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HepG2-Zellen, Mausherzgewebe, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hautgewebe, humanes Lebergewebe, humanes Nierengewebe, Rattenherzgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 28 publications below
• IHC: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14800-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA GOT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GOT2 fusion protein Ag6514
• Vollständiger Name: glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 43-45 kDa
• GenBank-Zugangsnummer: BC000525
• Gene symbol: GOT2
• Gene ID (NCBI): 2806
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.

Publikationen

Species	Application	Title
mouse	WB	Cell Stem Cell; Aspartate availability limits hematopoietic stem cell function during hematopoietic regeneration.; Authors - Le Qi ; KO Validated
	WB	Cell Metab; Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.; Authors - Qiuying Chen
human	WB,CoIP	Cell Metab; Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.; Authors - Yi-Ping Wang
mouse	WB	Cell Res; Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.; Authors - Wei-Feng Cai ; KD Validated
human	WB	Nat Commun; Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.; Authors - Yun-Qian Wang
human	WB,IHC	Nat Commun; Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.; Authors - Maren Feist

Verwandte Produkte

GOT1 Polyclonal antibody

14886-1-AP

MDH2 Polyclonal antibody

15462-1-AP

GLUD1 Polyclonal antibody

14299-1-AP

LDHC-Specific Polyclonal antibody

19989-1-AP

GPT / ALT1 Polyclonal antibody

16897-1-AP