CD42b Polyklonaler Antikörper

CD42b Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(31)

Kat-Nr. : 12860-1-AP

Synonyme

Antigen CD42b-alpha, BSS, CD42b alpha, GP1B, GP1BA

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  WB analysis of human peripheral blood platelets using 12860-1-AP

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 12860-1-AP (CD42b antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig peripheral blood leukocyte using 12860-1-AP

  pig peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 12860-1-AP (CD42b antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 12860-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 12860-1-AP (CD42b antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Human peripheral blood platelets, pig peripheral blood leukocyte, human blood

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 16 publications below
• IHC: See 12 publications below
• IF: See 3 publications below

Produktinformation

12860-1-AP bindet in WB, IHC, IF, ELISA CD42b und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD42b fusion protein Ag3893
• Vollständiger Name: glycoprotein Ib (platelet), alpha polypeptide
• Berechnetes Molekulargewicht: 626 aa, 69 kDa
• Beobachtetes Molekulargewicht: 145 kDa
• GenBank-Zugangsnummer: BC027955
• Gene symbol: CD42b
• Gene ID (NCBI): 2811
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GP1BA, also named as CD42b , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.

Publikationen

Species	Application	Title
human	WB	Adv Healthc Mater; Platelet Membrane-Fused Circulating Extracellular Vesicles Protect the Heart from Ischemia/Reperfusion Injury; Authors - Jizong Jiang
mouse	IF	Cancer Lett; Direct contact between tumor cells and platelets initiates a FAK-dependent F3/TGF-β positive feedback loop that promotes tumor progression and EMT in osteosarcoma; Authors - Qianyu Shi
rat	WB	Theranostics; A blood circulation-prolonging peptide anchored biomimetic phage-platelet hybrid nanoparticle system for prolonged blood circulation and optimized anti-bacterial performance.; Authors - Peipei Jin
human	WB	Int J Biol Macromol; Platelet-membrane camouflaged cerium nanoparticle-embedded gelatin methacryloyl hydrogel for accelerated diabetic wound healing; Authors - Hang Dong
mouse	WB	ACS Biomater Sci Eng; Biomimetic Platform Based on Mesoporous Platinum for Multisynergistic Cancer Therapy.; Authors - Gaoqian Zhao
mouse	IHC	Biochem Pharmacol; GSK669, a NOD2 receptor antagonist, inhibits thrombosis and oxidative stress via targeting platelet GPVI.; Authors - Guanxing Pan