CD42b Monoklonaler Antikörper

CD42b Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3G1F5

Kat-Nr. : 67857-1-Ig

Synonyme

GP1BA, GP1B, CD42b alpha, BSS, Antigen CD42b-alpha

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  WB analysis of human peripheral blood platelets using 67857-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67857-1-Ig (CD42b antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human blood using 67857-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 67857-1-Ig (CD42b antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 67857-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 67857-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 67857-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67857-1-Ig (CD42b antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human peripheral blood platelets, human blood
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67857-1-Ig bindet in WB, IHC, ELISA CD42b und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD42b fusion protein Ag31181
• Vollständiger Name: glycoprotein Ib (platelet), alpha polypeptide
• Berechnetes Molekulargewicht: 626 aa, 69 kDa
• Beobachtetes Molekulargewicht: 145 kDa
• GenBank-Zugangsnummer: BC027955
• Gene symbol: CD42b
• Gene ID (NCBI): 2811
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD42b, also named as GP1BA , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.