GPBP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GPBP1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 21622-1-AP

Synonyme

GPBP, GPBP1, SSH6, Vascular wall linked protein, VASCULIN

---

• 

  WB analysis of HepG2 using 21622-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21622-1-AP (GPBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21622-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21622-1-AP (GPBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 21622-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21622-1-AP (GPBP1 antibody) at dilution of 1:300 (under 10x lens).

• 

  IHC staining of human small intestine using 21622-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21622-1-AP (GPBP1 antibody) at dilution of 1:300 (under 40x lens).

• 

  IHC staining of human small intestine using 21622-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine slide using 21622-1-AP (GPBP1 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 21622-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 21622-1-AP (GPBP1 Antibody) at dilution of 1:50.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Produktinformation

21622-1-AP bindet in WB, IP, IHC, ELISA GPBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPBP1 fusion protein Ag14877
• Vollständiger Name: GC-rich promoter binding protein 1
• Berechnetes Molekulargewicht: 473 aa, 53 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC000267
• Gene symbol: GPBP1
• Gene ID (NCBI): 65056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IP	Exp Mol Med; Loss of RTN3 phenocopies chronic kidney disease and results in activation of the IGF2-JAK2 pathway in proximal tubular epithelial cells.; Authors - Liang-Liang Fan ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Joseph (Verified Customer) (04-30-2025)

Protein amount used for detection: 15–40 μg total protein per well Specificity: Validated with GPBP1 KO cells; some non-specific bands observed Molecular weight: Detects two bands around 70 kDa Blocking: 5% BSA in PBS + 0.1% Tween 20 (5% milk caused high background) Primary antibody: 1:1500, 1 h at RT Wash: 3–4 × 5 min with PBS + 0.1% Tween 20

• Applications: Western Blot
• Primary Antibody Dilution: 1/1500
• Cell Tissue Type: HCT116 and HEK293

GPBP1 Antibody Western Blot validation (1/1500 dilution) in HCT116 and HEK293 (Cat no:21622-1-AP)