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GPBP1L1 Polyklonaler Antikörper

GPBP1L1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17375-1-AP

Synonyme

GPBP1L1, RP11 767N6.1, SP192, Vasculin like protein 1

Filter:
  • Western Blot (WB) analysis of various lysates using GPBP1L1 Polyclonal antibody (17375-1-AP)

    WB analysis using 17375-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17375-1-AP (GPBP1L1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GPBP1L1 Polyclonal antibody (17375-1-AP)

    WB analysis of HepG2 using 17375-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17375-1-AP (GPBP1L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

17375-1-AP bindet in WB, ELISA GPBP1L1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen GPBP1L1 fusion protein Ag11180
Vollständiger Name GC-rich promoter binding protein 1-like 1
Berechnetes Molekulargewicht 474 aa, 52 kDa
Beobachtetes Molekulargewicht 58 kDa
GenBank-ZugangsnummerBC022218
Gene symbol GPBP1L1
Gene ID (NCBI) 60313
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for GPBP1L1 antibody 17375-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Joseph (Verified Customer) (04-30-2025)

Protein amount used for detection: 15–40 μg total protein per well Specificity: Validated with GPBP1L1 KO cells; some non-specific bands observed Molecular weight: Target band just below 70 kDa Blocking: 5% milk in PBS + 0.1% Tween 20 Primary antibody: 1:1500, 1 h at RT Wash: 3-4 × 5 min with PBS + 0.1% Tween 20

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1500
  • Cell Tissue Type: HCT116 and HEK293
GPBP1L1 Antibody Western Blot validation (1/1500 dilution) in HCT116 and HEK293 (Cat no:17375-1-AP)