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Glypican 4 Polyklonaler Antikörper
Glypican 4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 13048-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Maushirngewebe, Mausnierengewebe, Rattenhirngewebe |
| Erfolgreiche Detektion in IHC | humanes Magengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | U-251-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 3 publications below |
| WB | See 13 publications below |
| IHC | See 4 publications below |
| IF | See 5 publications below |
Produktinformation
13048-1-AP bindet in WB, IHC, IF/ICC, ELISA Glypican 4 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Glypican 4 fusion protein Ag3670 |
| Vollständiger Name | glypican 4 |
| Berechnetes Molekulargewicht | 556 aa, 62 kDa |
| Beobachtetes Molekulargewicht | 62 kDa, 30 kDa |
| GenBank-Zugangsnummer | BC017166 |
| Gene symbol | Glypican 4 |
| Gene ID (NCBI) | 2239 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Glypicans (GPCs) are a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation (PMID: 9931407). In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus (PMID: 10585884). They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Human GPC4 is a 556-amino acid protein, and shows 93.5% identity to mouse K-glypican. The gene of GPC4 has been mapped to Xq26, and its mRNA is widely expressed in human tissues, including lung, kidney, heart, placenta, skeletal muscle, and pancreas. GPC4 has also been shown to be present in astrocytes, haematopoietic-progenitor and bone-marrow-stromal cells. Studies suggest that GPC4 is involved in the regulation of Wnt signaling and astrocyte GPC4 plays a role in formation of excitatory synapses (PMID: 22302992; 22722203). This antobody can recognize both full length (65 kDa) and cleaved (35 kDa) GPC4 as well as GPC6 (PMID: 18055828; 22722203).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Glypican 4 antibody 13048-1-AP | Protokoll herunterladen |
| IHC protocol for Glypican 4 antibody 13048-1-AP | Protokoll herunterladenl |
| IF protocol for Glypican 4 antibody 13048-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nature Astrocyte glypicans 4 and 6 promote formation of excitatory synapses via GluA1 AMPA receptors.
| ||
Neuron Astrocyte-Secreted Glypican 4 Regulates Release of Neuronal Pentraxin 1 from Axons to Induce Functional Synapse Formation. | ||
Sci Adv Cocaine preference and neuroadaptations are maintained by astrocytic NMDA receptors in the nucleus accumbens. | ||
Nat Commun Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.
| ||
Nat Commun CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Dayana (Verified Customer) (06-16-2025) | I tested the GPC4 antibody in protein extracts from HEK293 overexpressing GPC4 or GPC6 and treated with shRNA. I can see clearly the overexpression of GPC4 , with no crossreaction with GPC6, however there are many non specific bands. I tested with the GPC4 overexpression after I failed to detect a 62 KDa band on primary neurons lysate. For westerblots, the samples were transfered in PVDF and blocked with 5% Milk TBST and the antibody incubated in 1% BSA TBST.
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FH Sara (Verified Customer) (05-26-2025) | Immunofluorescence: works well (red) on Gpc4-transfected cells (GFP, green)
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FH Rebecca (Verified Customer) (02-21-2022) | Western performed on Biorad MiniPROTEAN 4-15% TGX precast gels and Precision Plus All Blue Protein Ladder and Non-boil reducing buffer. Blocking performed for 1h at room temperature using 5%BLOTTO. Primary antibody dilution was performed overnight at 4°C in 5%BLOTTO/0.05%Tween. Secondary antibody was LiCOR IRDye® 680RD Donkey anti-Mouse IgG (H + L) at 1:20000 dilution in PBS-Tween-SDS as per manufacturers instructions for 2h at room temperature. Blot showed a clear band of the expected size with very low-none background/off target binding in the membrane fraction. Non-specific bands were observed in the cytosolic fraction.
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FH Elena (Verified Customer) (11-19-2018) |
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