GPD1 Polyklonaler Antikörper

GPD1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 13451-1-AP

Synonyme

cGPDH, EC:1.1.1.8, Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic, GPD 1, GPD C

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  WB analysis using 13451-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13451-1-AP (GPD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13451-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13451-1-AP (GPD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 13451-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13451-1-AP (GPD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 13451-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPD1 antibody (13451-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HepG2-Zellen, Mausherzgewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

13451-1-AP bindet in WB, IHC, IF/ICC, ELISA GPD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPD1 fusion protein Ag4278
• Vollständiger Name: glycerol-3-phosphate dehydrogenase 1 (soluble)
• Berechnetes Molekulargewicht: 349 aa, 38 kDa
• Beobachtetes Molekulargewicht: 32-42 kDa
• GenBank-Zugangsnummer: BC032234
• Gene symbol: GPD1
• Gene ID (NCBI): 2819
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPD1 (Glycerol-3-phosphate dehydrogenase 1) is an important enzyme belonging to the NAD-dependent glycerol-3-phosphate dehydrogenase family. Its C-terminal structural domain contains multiple helical structures for binding the substrate DHAP, and its N-terminal structural domain contains a β-folded core for binding NADH. GPD1 catalyzes the conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotinamide adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+, and plays a key role in carbohydrate and lipid metabolism. GPD1 also works with mitochondrial glycerol-3-phosphate dehydrogenase to form a glycerophosphate shuttle system that facilitates the transfer of reducing equivalents from the cytoplasm to the mitochondria. Abnormal activity of GPD1 has been associated with a variety of metabolic disorders, such as obesity, hypertriglyceridemia, and GPD1 has been implicated in cancer, potentially acting as a tumor suppressor.

Publikationen

Species	Application	Title
human	IHC	J Hematol Oncol; Allosteric activation of the metabolic enzyme GPD1 inhibits bladder cancer growth via the lysoPC-PAFR-TRPV2 axis.; Authors - Wenlong Zhang
human	WB	Proc Natl Acad Sci U S A; A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria.; Authors - Shiqi Wu
mouse	WB	J Ethnopharmacol; Si-Ni-San promotes liver regeneration by maintaining hepatic oxidative equilibrium and glucose/lipid metabolism homeostasis; Authors - Xu Yang
rat	WB	Mol Biol Rep; Glucose enhances catecholamine-stimulated lipolysis via increased glycerol-3-phosphate synthesis in 3T3-L1 adipocytes and rat adipose tissue.; Authors - Nodoka Takeuchi
		Int J Biol Macromol; Genistein and metformin regulate glycerol kinase and the enzymes of glycerol 3-phosphate shuttle in a differential manner in myocytes, hepatocytes and adipocytes; Authors - Superior Syngkli
mouse	WB	Phytomedicine; Astragaloside IV and cycloastragenol promote liver regeneration through regulation of hepatic oxidative homeostasis and glucose/lipid metabolism; Authors - Yanghao Li