GPI Polyklonaler Antikörper

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• KD/KO Validated

GPI Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(31)

Rezensionen (3)

Kat-Nr. : 15171-1-AP

Synonyme

NLK, Neuroleukin, GNPI, Glucose-6-phosphate isomerase, EC:5.3.1.9

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  WB analysis of HeLa using 15171-1-AP

  WB result of GPI antibody (15171-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells.

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  WB analysis using 15171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15171-1-AP (GPI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15171-1-AP (GPI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 15171-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15171-1-AP (GPI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15171-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15171-1-AP (GPI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15171-1-AP

  WB result of GPI antibody (15171-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells.

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  IHC staining of human lung cancer using 15171-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15171-1-AP (GPI Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15171-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15171-1-AP (GPI Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 15171-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15171-1-AP (GPI antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 15171-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GPI antibody (15171-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, PC3-Zellen, PC-3-Zellen, U251-Zellen, U87-MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, human normal colon; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 28 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

15171-1-AP bindet in WB, IHC, IF/ICC, ELISA GPI und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPI fusion protein Ag7423
• Vollständiger Name: glucose phosphate isomerase
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 55-64 kDa
• GenBank-Zugangsnummer: BC004982
• Gene symbol: GPI
• Gene ID (NCBI): 2821
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).

Publikationen

Species	Application	Title
human	WB	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
mouse	IF	Nat Neurosci; A glycolytic shift in Schwann cells supports injured axons.; Authors - Elisabetta Babetto
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
mouse	WB	EMBO J; Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.; Authors - Lihua Wang
human	WB	EMBO Rep; CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.; Authors - Xiuying Zhong
human	WB	EMBO Rep; OMA1 reprograms metabolism under hypoxia to promote colorectal cancer development.; Authors - Zhida Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marco (Verified Customer) (07-04-2024)

A great antibody for NRVCMs

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NRVCMs

FH

Kristian (Verified Customer) (07-26-2021)

GPI is shown in green. Nuclei are shown in blue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: RCC4

GPI Antibody Immunofluorescence validation (1:200 dilution) in RCC4 (Cat no:15171-1-AP)

FH

Daniel (Verified Customer) (02-06-2020)

Denatured lysate was separated on a 4-20% PAGE

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse kidney

GPI Antibody Western Blot, validation (1:2000 dilution) in mouse kidney (Cat no:15171-1-AP)