GPR108 Polyklonaler Antikörper

GPR108 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 24009-1-AP

Synonyme

G protein coupled receptor 108, GPR108, LUSTR2, Protein GPR108

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  WB analysis of mouse kidney using 24009-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24009-1-AP (GPR108 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 24009-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 24009-1-AP (GPR108 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 24009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24009-1-AP (GPR108 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 24009-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24009-1-AP (GPR108 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 24009-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24009-1-AP (GPR108 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

24009-1-AP bindet in WB, IF, IHC, ELISA GPR108 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPR108 fusion protein Ag20887
• Vollständiger Name: G protein-coupled receptor 108
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 61-70 kDa
• GenBank-Zugangsnummer: BC007862
• Gene symbol: GPR108
• Gene ID (NCBI): 56927
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPR108 is a seven-transmembrane family protein that falls in the GPCRs superfamily (PMID:30332431). GPR108 was identified as a highly conserved AAV entry factor implicated in cellular immune responses to AAV (PMID:31784416). Of note is its role in activating nuclear factor kappa-B (NF-κB) signaling, which implied the potential involvement of GPR108 in inflammation-related diseases as well as cancers through dysregulating NF-κB activity (PMID:35659621).

Publikationen

Species	Application	Title
human	WB,IF	J Virol; Interplay between Furin and Sialoglycans in Modulating Adeno-Associated Viral Cell Entry; Authors - Timothy J Smith
human	WB	Nat Chem Biol; Chemoproteomics reveals microbiota-derived aromatic monoamine agonists for GPRC5A; Authors - Xiaohui Zhao