GPR132 Polyklonaler Antikörper

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• KD/KO Validated

GPR132 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 17026-1-AP

Synonyme

G protein coupled receptor 132, G2 accumulation protein, G2A, GPR132

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Galerie der Validierungsdaten

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  IHC staining of human kidney using 17026-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17026-1-AP (GPR132 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 17026-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17026-1-AP (GPR132 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 17026-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17026-1-AP (GPR132 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17026-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17026-1-AP (GPR132 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• IHC: See 1 publications below

Produktinformation

17026-1-AP bindet in IHC, ELISA GPR132 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPR132 fusion protein Ag10325
• Vollständiger Name: G protein-coupled receptor 132
• Berechnetes Molekulargewicht: 380 aa, 43 kDa
• GenBank-Zugangsnummer: BC084546
• Gene symbol: GPR132
• Gene ID (NCBI): 29933
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPR132, also known as G2A (G2 accumulation protein), belongs to the G-protein coupled receptor (GPCR) 1 family. GPR132 is a DNA damage and stress inducible GPCR that blocks cells in G2/M. It functions at the G2/M checkpoint to delay mitosis and may serve as a mechanism for T and B cells and other cell types to slow their proliferation and repair damaged DNA to ensure proper replication (PMID: 9770487). Lysophosphatidylcholine (LPC) is a high affinity ligand for GPR132 (PMID: 15653487).

Publikationen

Species	Application	Title
human	IHC	J Eur Acad Dermatol Venereol; Lysophosphatidylcholine facilitates the pathogenesis of psoriasis through activating keratinocytes and T cells differentiation via glycolysis; Authors - Panpan Liu
mouse		Front Oncol; Lactate increases tumor malignancy by promoting tumor small extracellular vesicles production via the GPR81-cAMP-PKA-HIF-1α axis; Authors - Man Luo ; KD Validated
mouse		Neurosci Res; Proton-sensing receptor GPR132 facilitates migration of astrocytes.; Authors - Ken Matoba
human		J Hepatobiliary Pancreat Sci; Partial characterization of proapoptotic action of biliary deteriorated lipids on biliary epithelial cells in pancreaticobiliary diseases.; Authors - Fujita Keiko K
mouse		Nat Commun; Lysophosphatidylserine induces necrosis in pressure overloaded male mouse hearts via G protein coupled receptor 34; Authors - Ryuta Sugihara