GRASP65,GORASP1 Monoklonaler Antikörper

GRASP65,GORASP1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1B2C10

Kat-Nr. : 66651-1-Ig

Synonyme

GORASP1, 1B2C10, Golgi phosphoprotein 5, Golgi reassembly-stacking protein 1, Golgi reassembly-stacking protein of 65 kDa

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  WB analysis using 66651-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66651-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66651-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66651-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 66651-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 66651-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SK-BR-3 using 66651-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SK-BR-3 cells using GRASP65,GORASP1 antibody (66651-1-Ig, Clone: 1B2C10 ) at dilution of 1:3876 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SK-BR-3 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1938-1:7752

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

66651-1-Ig bindet in WB, IHC, IF/ICC, ELISA GRASP65,GORASP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GRASP65,GORASP1 fusion protein Ag1054
• Vollständiger Name: golgi reassembly stacking protein 1, 65kDa
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC008928
• Gene symbol: GRASP65
• Gene ID (NCBI): 64689
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum.

Publikationen

Species	Application	Title
human	WB	Front Cell Dev Biol; Autophagy-Based Unconventional Secretory for AIM2 Inflammasome Drives DNA Damage Resistance During Intervertebral Disc Degeneration.; Authors - Shuai Li
human	WB	Mol Biol Cell; Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.; Authors - Stephen C Ireland
human	WB	J Cell Biochem; Golgi apparatus fragmentation participates in oxidized low-density lipoprotein-induced endothelial cell injury.; Authors - Guang Ji
human	WB	Cells; Discovery of Cell Number-Interstitial Fluid Volume (CIF) Ratio Reveals Secretory Autophagy Pathway to Supply eHsp90α for Wound Healing; Authors - Cheng Chang ; KO Validated