GRB10 Polyklonaler Antikörper

GRB10 Polyklonal Antikörper für WB, IF, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16622-1-AP

Synonyme

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  WB analysis of HepG2 using 16622-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16622-1-AP (GRB10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16622-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16622-1-AP (GRB10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 16622-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16622-1-AP (GRB10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16622-1-AP bindet in WB, IF, ELISA GRB10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRB10 fusion protein Ag9921
• Vollständiger Name: growth factor receptor-bound protein 10
• Berechnetes Molekulargewicht: 536aa,61 kDa; 594aa,67 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC024285
• Gene symbol: GRB10
• Gene ID (NCBI): 2887
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Growth factor receptor-bound protein 10 (GRB10) is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including the ins (INSR) and ins-like growth factor (IGF1R) receptors. It may play a role in mediating ins-stimulated ubiquitination of INSR, leading to proteasomal degradation.