Glutamate receptor 2 Polyklonaler Antikörper

Glutamate receptor 2 Polyklonal Antikörper für FC (Intra), IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC (Intra), CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

31

Rezensionen (1)

Kat-Nr. : 11994-1-AP

Synonyme

GluA2, GluR 2, GluR B, GluR K2, GLUR2, GLURB, Glutamate receptor 2, GRIA2, HBGR2

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 11994-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11994-1-AP (Glutamate receptor 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11994-1-AP

  IP Result of anti-Glutamate receptor 2 (IP:11994-1-AP, 4ug; Detection:11994-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 11994-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11994-1-AP (Glutamate receptor 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse eye using 11994-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Glutamate receptor 2 antibody (11994-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 11994-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug Glutamate receptor 2 antibody (11994-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

• 

  FC experiment of SH-SY5Y using 11994-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Glutamate receptor 2 (11994-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Augengewebe
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 27 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

11994-1-AP bindet in WB, IP, IHC, IF, FC (Intra), CoIP, ELISA Glutamate receptor 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Glutamate receptor 2 fusion protein Ag2662
• Vollständiger Name: glutamate receptor, ionotropic, AMPA 2
• Berechnetes Molekulargewicht: 883 aa, 99 kDa
• Beobachtetes Molekulargewicht: 94-105 kDa
• GenBank-Zugangsnummer: BC010574
• Gene symbol: GRIA2
• Gene ID (NCBI): 2891
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. GRIA2 (glutamate receptor 2, GluR-2) belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels that mediate the fast component of excitatory postsynaptic currents in neurons of the central nervous system. These channels are assembled from 4 related subunits, GRIA1-4. The presence of GRIA2 in the heteromeric AMPA receptors impermeabilizes it to calcium, preventing possible calcium-mediated toxicity.

Publikationen

Species	Application	Title
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
mouse,human	WB,CoIP	Sci Adv; RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission; Authors - Pengcheng Ma
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin
human	WB	J Clin Invest; TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.; Authors - Haiqing Zhang
mouse	WB	Mol Neurobiol; A Regulatory Circuitry Between Gria2, miR-409, and miR-495 Is Affected by ALS FUS Mutation in ESC-Derived Motor Neurons.; Authors - Davide Capauto
mouse	IP	Cell Death Dis; ZDHHC8 critically regulates seizure susceptibility in epilepsy.; Authors - Qin Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Lisa (Verified Customer) (10-04-2021)

The antibody signal is quite strong (50-70sec of exposure) and gives "clean" bands. The diluted antibody (TBS 0.1% Tween+5% BSA) does not work well anymore after 2-3 times of re-use

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSCs-derived neurons

Verwandte Produkte

GRIA4 Polyclonal antibody

23350-1-AP

CACNG2 Polyclonal antibody

20969-1-AP

CACNG3 Polyclonal antibody

13729-1-AP

GRIK2 Polyclonal antibody

13597-1-AP

GRIP1 Polyclonal antibody

22398-1-AP