GRK5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GRK5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 17032-1-AP

Synonyme

GPRK5, GRK5

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  WB analysis of human skeletal muscle using 17032-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17032-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 17032-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17032-1-AP (GRK5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 17032-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17032-1-AP (GRK5 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 17032-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17032-1-AP (GRK5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse heart using 17032-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17032-1-AP (GRK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 17032-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17032-1-AP (GRK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

17032-1-AP bindet in WB, IHC, IF, ELISA GRK5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRK5 fusion protein Ag10511
• Vollständiger Name: G protein-coupled receptor kinase 5
• Berechnetes Molekulargewicht: 590 aa, 68 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC064506
• Gene symbol: GRK5
• Gene ID (NCBI): 2869
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRK5, also named as GPRK5, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. It is Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. GRK5 is crucial for proper neuronal morphogenesis and the establishment of functional neuronal circuitry.

Publikationen

Species	Application	Title
mouse,rat	WB,IF	Biomaterials; Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure; Authors - Lingjun Tong
human,mouse	IHC,IF	Arthritis Rheumatol; GRK5 Inhibition Attenuates Cartilage Degradation via Decreased NF-κB Signaling.; Authors - Takuya Sueishi ; KO Validated
mouse	WB	Cell Rep; METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling; Authors - Shuhua Luo
human	IHC	Front Mol Biosci; Co-Overexpression of GRK5/ACTC1 Correlates With the Clinical Parameters and Poor Prognosis of Epithelial Ovarian Cancer.; Authors - Longyang Liu
	WB	Biochim Biophys Acta Mol Cell Res; Ligand-dependent internalization of Bombyx mori tachykinin-related peptide receptor is regulated by PKC, GRK5 and β-arrestin2/BmKurtz.; Authors - Xiaobai He
human	IHC,IF	Sci Rep; G protein-coupled receptor kinase 5 deletion suppresses synovial inflammation in a murine model of collagen antibody-induced arthritis.; Authors - Masakazu Toya