GRLF1 Polyklonaler Antikörper

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• KD/KO Validated

GRLF1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 26789-1-AP

Synonyme

ARHGAP35, GRF 1, GRF1, GRLF1, KIAA1722, P190 A, P190A, p190RhoGAP, Rho GAP p190A

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Galerie der Validierungsdaten

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  WB analysis of A549 using 26789-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26789-1-AP (GRLF1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26789-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26789-1-AP (GRLF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 26789-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26789-1-AP (GRLF1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 26789-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26789-1-AP (GRLF1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 26789-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26789-1-AP (GRLF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 26789-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26789-1-AP (GRLF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

26789-1-AP bindet in WB, IHC, IF, ELISA GRLF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRLF1 fusion protein Ag25353
• Vollständiger Name: glucocorticoid receptor DNA binding factor 1
• Beobachtetes Molekulargewicht: 172 kDa
• GenBank-Zugangsnummer: BC150257
• Gene symbol: GRLF1
• Gene ID (NCBI): 2909
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRLF1 is also named as ARHGAP35, p190ARHOGAP, KIAA1722, P190A and GRF1. GRLF1 is a kind of Rho GTPase-activating protein, and it binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PMID:19673492). This binding is inhibited by phosphorylation by PRKCA (PMID:19673492). GRLF1 is Involved in cell differentiation as well as cell adhesion and migration. And it plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis.

Publikationen

Species	Application	Title
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu ; KD Validated
human	WB,IF	Oncogene; Ubiquitin ligase TRIM65 promotes colorectal cancer metastasis by targeting ARHGAP35 for protein degradation.; Authors - Daici Chen
human	WB	Front Cell Dev Biol; Feedback-Driven Mechanisms Between Phosphorylated Caveolin-1 and Contractile Actin Assemblies Instruct Persistent Cell Migration.; Authors - Xuemeng Shi