mGluR2 Polyklonaler Antikörper

mGluR2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 19956-1-AP

Synonyme

GRM2, GLUR2, GPRC1B, mGlu2

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  WB analysis of rat brain using 19956-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 19956-1-AP (mGluR2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 19956-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19956-1-AP (mGluR2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 19956-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19956-1-AP (mGluR2 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 19956-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19956-1-AP (mGluR2 antibody at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

19956-1-AP bindet in WB, IHC, ELISA mGluR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: glutamate receptor, metabotropic 2
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 96 kDa, 120 kDa
• GenBank-Zugangsnummer: NM_000839
• Gene symbol: mGluR2
• Gene ID (NCBI): 2912
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRM2, also named GPRC1B and MGLUR2, belongs to the G-protein coupled receptor 3 families. GRM2 is a receptor for glutamate. The activity of GRM2 is mediated by a G-protein that inhibits adenylate cyclase activity. It may mediate the suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.

Publikationen

Species	Application	Title
human	WB	Mol Cancer Res; Long-range epigenetic silencing associates with deregulation of Ikaros targets in colorectal cancer cells.; Authors - Javierre Biola M BM
rat	WB,IHC	Auton Neurosci; Expression of group II metabotropic glutamate receptors in rat superior cervical ganglion; Authors - Xixi Wei
rat	WB,IHC	Neurochem Int; Red nucleus mGluR2 but not mGluR3 mediates inhibitory effect in the development of SNI-induced neuropathological pain by suppressing the expressions of TNF-α and IL-1β; Authors - Wen-Tao Wang