GRP78/BIP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GRP78/BIP Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (5)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

545

Rezensionen (7)

Kat-Nr. : 11587-1-AP

Synonyme

BIP, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSP A5, HSPA5, MIF2

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Galerie der Validierungsdaten

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  WB analysis using 11587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11587-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11587-1-AP (GRP78/BIP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 11587-1-AP

  IP Result of anti-GRP78/BIP (IP:11587-1-AP, 3ug; Detection:11587-1-AP 1:1000) with MCF-7 cells lysate 3250ug.

• 

  IHC staining of human pancreas cancer using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat brain using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse kidney using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11587-1-AP (GRP78/BIP antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 11587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11587-1-AP (GRP78/BIP antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11587-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRP78/BIP antibody (11587-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 11587-1-AP

  Immunofluorescent analysis of HepG2 cells using 11587-1-AP (GRP78/BIP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of HeLa using 11587-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GRP78/BIP (11587-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, Caco-2-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HT-29-Zellen, Maushirngewebe, Mauslebergewebe, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe, Mausherzgewebe, Mausnierengewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 15 publications below
• WB: See 488 publications below
• IHC: See 70 publications below
• IF: See 75 publications below
• IP: See 11 publications below
• CoIP: See 5 publications below

Produktinformation

11587-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA GRP78/BIP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Fisch, Huhn, Maus, Ratte, Zebrafisch, Medaka-Embryos
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRP78/BIP fusion protein Ag2188
• Vollständiger Name: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 70-78 kDa
• GenBank-Zugangsnummer: BC020235
• Gene symbol: HSPA5
• Gene ID (NCBI): 3309
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.

Publikationen

Species	Application	Title
	WB	Science; Translation from the 5' untranslated region shapes the integrated stress response.; Authors - Shelley R Starck
mouse	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
mouse	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
mouse	WB	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
human,rat	WB	Acta Pharm Sin B; SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation.; Authors - Fen Long
human,mouse	WB	J Pineal Res; Melatonin alleviates PM2.5 -induced glucose metabolism disorder and lipidome alteration by regulating endoplasmic reticulum stress; Authors - Zhou Du

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

PK (Verified Customer) (03-22-2022)

• Applications: Western Blot
• Cell Tissue Type: FIBROBLAST

GRP78/BIP Antibody Western Blot validation ( dilution) in FIBROBLAST (Cat no:11587-1-AP)

FH

Yeawon (Verified Customer) (03-17-2022)

Works very good in mouse kidney tissue.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse kidney tissue

FH

Emma (Verified Customer) (03-15-2022)

Works well by WB in LNCaP and CWR22Rv1 cells @ 1:1000. A single band observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Prostate

FH

P (Verified Customer) (05-27-2021)

Excellent.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: H9C2

GRP78/BIP Antibody Western Blot validation (1:2000 dilution) in H9C2 (Cat no:11587-1-AP)

FH

Kevin (Verified Customer) (01-24-2019)

Works pretty good in HepG2 cells. One single band was observed.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2

FH

Sylvain (Verified Customer) (12-03-2018)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: colon cancer cell lines

FH

Natalie (Verified Customer) (11-20-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Rat Dorsal Root Ganglia

GRP78/BIP Antibody Western Blot, validation (1:5000 dilution) in Rat Dorsal Root Ganglia (Cat no:11587-1-AP)

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