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GSDMD Polyklonaler Antikörper
GSDMD Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (5)
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 20770-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | A431-Zellen, C6-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, Rattenhirngewebe, Rattenlebergewebe |
| Erfolgreiche IP | THP-1-Zellen |
| Erfolgreiche Detektion in IHC | human ovary cancer tissue, Mauslungengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HeLa-Zellen |
| Erfolgreiche Detektion in FC (Intra) | Jurkat-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 7 publications below |
| WB | See 296 publications below |
| IHC | See 74 publications below |
| IF | See 100 publications below |
| IP | See 1 publications below |
Produktinformation
20770-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA GSDMD und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, hamster, Hausschwein, Kaninchen, Maus, Ratte, Rind, Zebrafisch |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | GSDMD fusion protein Ag14757 |
| Vollständiger Name | gasdermin D |
| Berechnetes Molekulargewicht | 484 aa, 53 kDa |
| Beobachtetes Molekulargewicht | 50-53 kDa |
| GenBank-Zugangsnummer | BC008904 |
| Gene symbol | GSDMD |
| Gene ID (NCBI) | 79792 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for GSDMD antibody 20770-1-AP | Protokoll herunterladen |
| IHC protocol for GSDMD antibody 20770-1-AP | Protokoll herunterladenl |
| IF protocol for GSDMD antibody 20770-1-AP | Protokoll herunterladen |
| IP protocol for GSDMD antibody 20770-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Cell Type III interferons induce pyroptosis in gut epithelial cells and impair mucosal repair | ||
Nature Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.
| ||
ACS Nano Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration. | ||
Acta Pharm Sin B Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis. | ||
Acta Pharm Sin B A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer | ||
Cell Res Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Isha (Verified Customer) (03-02-2021) | Bands were so clear. Could not refrain myself from writing this review.
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