GSDMD Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GSDMD Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (5)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(376)

Rezensionen (1)

Kat-Nr. : 20770-1-AP

Synonyme

FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD, Gasdermin-D

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  WB analysis of Jurkat using 20770-1-AP

  WB result of GSDMD antibody (20770-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSDMD transfected Jurkat cells.

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  WB analysis using 20770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20770-1-AP (GSDMD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20770-1-AP (GSDMD antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of THP-1 using 20770-1-AP

  IP result of anti-GSDMD (IP:20770-1-AP, 4ug; Detection:20770-1-AP 1:4000) with THP-1 cells lysate 1560 ug.

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  IHC staining of human ovary cancer using 20770-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20770-1-AP (GSDMD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 20770-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20770-1-AP (GSDMD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 20770-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20770-1-AP (GSDMD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 20770-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20770-1-AP (GSDMD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20770-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSDMD antibody (20770-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 20770-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSDMD antibody (20770-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of Jurkat using 20770-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug GSDMD Polyclonal antibody (20770-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, C6-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche IP: THP-1-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 296 publications below
• IHC: See 74 publications below
• IF: See 100 publications below
• IP: See 1 publications below

Produktinformation

20770-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA GSDMD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Kaninchen, Maus, Ratte, Rind, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSDMD fusion protein Ag14757
• Vollständiger Name: gasdermin D
• Berechnetes Molekulargewicht: 484 aa, 53 kDa
• Beobachtetes Molekulargewicht: 50-53 kDa
• GenBank-Zugangsnummer: BC008904
• Gene symbol: GSDMD
• Gene ID (NCBI): 79792
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.

Publikationen

Species	Application	Title
human	WB	Cell; Type III interferons induce pyroptosis in gut epithelial cells and impair mucosal repair; Authors - Kautilya K Jena
human	WB	Nature; Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.; Authors - Xing Liu ; KD Validated
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao
human	WB	Acta Pharm Sin B; Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.; Authors - Xiaowei Zhang
human	WB	Acta Pharm Sin B; A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer; Authors - Yang Liu
Human	WB	Cell Res; Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion.; Authors - Wan-Ting He

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Isha (Verified Customer) (03-02-2021)

Bands were so clear. Could not refrain myself from writing this review.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mice kidney