GSDMD Rekombinanter Antikörper

GSDMD Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

2N24

Kat-Nr. : 80918-1-RR

Synonyme

2N24, FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD

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  WB analysis using 80918-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80918-1-RR (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of RAW 264.7 using 80918-1-RR

  1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human GSDMD (80918-1-RR, Clone:2N24) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 80918-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80918-1-RR against Human GSDMD were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in FC (Intra): RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80918-1-RR bindet in WB, FC (Intra), ELISA GSDMD und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GSDMD fusion protein Ag14757
• Vollständiger Name: gasdermin D
• Berechnetes Molekulargewicht: 484 aa, 53 kDa
• Beobachtetes Molekulargewicht: 50-53 kDa
• GenBank-Zugangsnummer: BC008904
• Gene symbol: GSDMD
• Gene ID (NCBI): 79792
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.