GSS Polyklonaler Antikörper

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• KD/KO Validated

GSS Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

hamster, Hausschwein, human, Hund, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(24)

Rezensionen (1)

Kat-Nr. : 15712-1-AP

Synonyme

GSHS, GSH synthetase, GSH S, glutathione synthetase, Glutathione synthase

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  WB analysis using 15712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15712-1-AP (GSS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15712-1-AP

  WB result of GSS antibody (15712-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSS transfected HEK-293 cells.

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  IHC staining of human kidney using 15712-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15712-1-AP (GSS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 15712-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15712-1-AP (GSS Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MDCK using 15712-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 15712-1-AP (GSS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: CHO-Zellen, HEK-293-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 22 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

15712-1-AP bindet in WB, IHC, IF/ICC, ELISA GSS und zeigt Reaktivität mit hamster, Hausschwein, human, Hund, Maus, Ratten

• Getestete Reaktivität: hamster, Hausschwein, human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSS fusion protein Ag8338
• Vollständiger Name: glutathione synthetase
• Berechnetes Molekulargewicht: 474 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC007927
• Gene symbol: GSS
• Gene ID (NCBI): 2937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse,human	WB,IHC,IF	ACS Nano; Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma; Authors - Xiao Liu
human	WB	Adv Sci (Weinh); Paracrine Orchestration of Tumor Microenvironment Remodeling Induced by GLO1 Potentiates Lymph Node Metastasis in Breast Cancer; Authors - Jindong Xie
human	WB	Oxid Med Cell Longev; IMCA Induces Ferroptosis Mediated by SLC7A11 through the AMPK/mTOR Pathway in Colorectal Cancer.; Authors - Lei Zhang
mouse	WB	Redox Biol; γ-glutamylcysteine exhibits anti-inflammatory effects by increasing cellular glutathione level.; Authors - Yang Yang
human	WB	Pharmacol Res; Phyto-sesquiterpene lactones DET and DETD-35 induce ferroptosis in vemurafenib sensitive and resistant melanoma via GPX4 inhibition and metabolic reprogramming.; Authors - Meng-Ting Chang
mouse	WB	FASEB J; Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.; Authors - Kenneth N Maclean

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (08-05-2021)

This is the second tube I purchased from ProteinTech. I got my first tube of this antibody in 2016 and it worked well. However, it suddenly stopped working last year. There is no signal at all on the membrane. Thus, I purchased the second tube in May 2021 and I just used it on my experiments as my previous record showing it is working well in WB. Unfortunately, I couldn't get any signal at all. I ran two tests with this antibody and it shows it stopping work with mouse liver.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human liver, mouse liver, mouse brain and mouse kidney.

GSS Antibody Western Blot validation (1:1000 dilution) in Human liver, mouse liver, mouse brain and mouse kidney. (Cat no:15712-1-AP)