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GST Tag Polyklonaler Antikörper

GST Tag Polyklonal Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Kat-Nr. : CL647-10000

Synonyme

GST, Rabbit IgG control

Galerie der Validierungsdaten

Filter:
  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated GST Tag Polyclonal a (CL647-10000)

    FC experiment of HeLa using CL647-10000

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-N/A GST Tag (CL647-10000) (blue), or 0.4 ug CoraLite®488-conjugated TRAP1 Rabbit PolyAb (CL488-10325, Clone: ) (red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-10000 bindet in FC (Intra) GST Tag und zeigt Reaktivität mit rekombinanten Protein

Getestete Reaktivität rekombinanten Protein
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name GST Tag
Berechnetes Molekulargewicht 26 kDa
GenBank-ZugangsnummerFN315687
Gene symbol
Gene ID (NCBI)
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Antigen-affinitätsgereinigt
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 647 GST Tag antibody CL647-10000Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen