GST Tag Polyklonaler Antikörper

GST Tag Polyklonal Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Publikationen(1)

Kat-Nr. : CL647-10000

Synonyme

Rabbit IgG control, GST, Glutathione S-Transferase

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  FC experiment of Jurkat using CL647-10000

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-GST Tag (CL647-10000) (red), or 0.4 ug CoraLite® Plus 488-conjugated IGF2R antibody (CL488-20253) (green), or 0.4 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-10000 bindet in FC (Intra) GST Tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: GST Tag
• Berechnetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: FN315687
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.

Publikationen

Species	Application	Title
		Viruses; Tumor Necrosis Factor and Interleukin-1β Upregulate NRP2 Expression and Promote SARS-CoV-2 Proliferation; Authors - Michinori Ishitoku