GSTA1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GSTA1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(22)

Kat-Nr. : 14475-1-AP

Synonyme

Glutathione S transferase A1, EC:5.3.3.-, EC:2.5.1.18, EC:1.11.1.-, Androst-5-ene-3,17-dione isomerase

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  WB analysis using 14475-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14475-1-AP (GSTA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14475-1-AP

  WB result of GSTA1 antibody (14475-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTA1 transfected HepG2 cells.

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  WB analysis of human kidney using 14475-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14475-1-AP (GSTA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14475-1-AP

  IP result of anti-GSTA1 (IP:14475-1-AP, 3ug; Detection:14475-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human lung cancer using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14475-1-AP (GSTA1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 14475-1-AP (GSTA1 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 14475-1-AP (GSTA1 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 14475-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 14475-1-AP (GSTA1 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 14475-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GSTA1 antibody (14475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HepG2-Zellen, humanes Nierengewebe, Mauslebergewebe, Rattennierengewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Nierengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 19 publications below
• IHC: See 3 publications below

Produktinformation

14475-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA GSTA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSTA1 fusion protein Ag5849
• Vollständiger Name: glutathione S-transferase alpha 1
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC053578
• Gene symbol: GSTA1
• Gene ID (NCBI): 2938
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GSTA1 encodes one of the GST alpha isozymes, belongs to GST family, which is mainly expressed in the liver. GST can improve the detoxification of organism by enzymatic and non-enzymatic methods, and has anti-mutagenic and anti-tumor effects. Studies have shown that overexpression of GSTA1 can promote the development of lung cancer cells, so the expression level of GSTA1 can be used to predict the degree of malignancy of lung cancer tissues. To some extent, GSTA1 is regarded as a potential lung cancer candidate targets.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions; Authors - Shengjie Fan
		Sci Adv; Treatment with siRNAs is commonly associated with GPX4 up-regulation and target knockdown-independent sensitization to ferroptosis; Authors - Anne von Mässenhausen
mouse	WB	Cell Death Dis; Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.; Authors - Cheng Wang
mouse	WB	Toxicology; Loss of Urat1 exacerbates APAP-induced liver injury in mice; Authors - Kunlu Zhao
mouse	WB	J Gerontol A Biol Sci Med Sci; PMID: 24285747; Authors - Rojanathammanee Lalida L
mouse	WB	Age (Dordr); PMID: 25001375; Authors - Lalida Rojanathammanee