GSTP1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

GSTP1 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

CloneNo.

2D4A6

Kat-Nr. : 66715-1-Ig

Synonyme

2D4A6, EC:2.5.1.18, FAEES3, Glutathione S-transferase P, GST3

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  WB analysis using 66715-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66715-1-Ig (GSTP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66715-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66715-1-Ig (GSTP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66715-1-Ig

  WB result of GSTP1 antibody (66715-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTP1 transfected HeLa cells.

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  IP experiment of HeLa using 66715-1-Ig

  IP result of anti-GSTP1 (IP:66715-1-Ig, 4ug; Detection:66715-1-Ig 1:4000) with HeLa cells lysate 1640 ug.

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  IHC staining of human colon cancer using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66715-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GSTP1 antibody (66715-1-Ig, Clone: 2D4A6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66715-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSTP1 antibody (66715-1-Ig, Clone: 2D4A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HuH-7-Zellen, Caco-2-Zellen, HCT 116-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Zervixkarzinomgewebe, humanes Nierengewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

66715-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA GSTP1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GSTP1 fusion protein Ag8880
• Vollständiger Name: glutathione S-transferase pi 1
• Berechnetes Molekulargewicht: 210 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC010915
• Gene symbol: GSTP1
• Gene ID (NCBI): 2950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Biochem Pharmacol; PMID: 32535103; Authors - Qi Feng ; KD Validated
human	WB	Mol Med Rep; Targeted reversal of multidrug resistance in ovarian cancer cells using exosome‑encapsulated tetramethylpyrazine; Authors - Chenge Zhao
human	IF	Phytomedicine; Natural small-molecules reverse Xeroderma Pigmentosum Complementation Group C (XPC) deficient-mediated drug-resistance in renal cell carcinoma; Authors - Ruihong Chen
mouse	CoIP	Eur J Pharmacol; N6022 attenuates cerebral ischemia/reperfusion injury-induced microglia ferroptosis by promoting Nrf2 nuclear translocation and inhibiting the GSNOR/GSTP1 axis; Authors - Wan-Li Duan
Human		Onco Targets Ther; Mechanism of microRNA-152-3p-Mediated Regulation of Autophagy and Sensitivity in Paclitaxel-Resistant Ovarian Cancer Cells; Authors - Di Wu
human	WB	Bioorg Chem; Sesquiterpene lactones from Elephantopus scaber exhibit cytotoxic effects on glioma cells by targeting GSTP1; Authors - Qiu-Lin Yan