GSTP1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

GSTP1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

2D4A6

Kat-Nr. : 66715-1-Ig

Synonyme

DFN7, FAEES3, Glutathione S transferase P, glutathione S transferase pi 1, GST class pi, GST3, GSTP1, GSTP1 1, PI

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Galerie der Validierungsdaten

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  WB analysis using 66715-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66715-1-Ig (GSTP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66715-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66715-1-Ig (GSTP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66715-1-Ig

  WB result of GSTP1 antibody (66715-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTP1 transfected HeLa cells.

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  IHC staining of human colon cancer using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66715-1-Ig (GSTP1 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66715-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GSTP1 antibody (66715-1-Ig, Clone: 2D4A6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66715-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSTP1 antibody (66715-1-Ig, Clone: 2D4A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Ovarialkarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below

Produktinformation

66715-1-Ig bindet in WB, IHC, IF, ELISA GSTP1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GSTP1 fusion protein Ag8880
• Vollständiger Name: glutathione S-transferase pi 1
• Berechnetes Molekulargewicht: 210 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC010915
• Gene symbol: GSTP1
• Gene ID (NCBI): 2950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Biochem Pharmacol; PMID: 32535103; Authors - Qi Feng ; KD Validated
human	WB	Bioorg Chem; Sesquiterpene lactones from Elephantopus scaber exhibit cytotoxic effects on glioma cells by targeting GSTP1; Authors - Qiu-Lin Yan
rat	WB	Free Radic Biol Med; Ginsenoside Rg1 protects cardiac mitochondrial function via targeting GSTP1 to block S-glutathionylation of optic atrophy 1; Authors - Huimin Zhu
human	WB	Cell Mol Life Sci; NUMA1 modulates apoptosis of esophageal squamous cell carcinoma cells through regulating ASK1-JNK signaling pathway; Authors - Shuying Yin ; KD Validated