GTF2F1 Polyklonaler Antikörper

GTF2F1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10093-2-AP

Synonyme

BTF4, GTF2F1, RAP74, TF2F1, TFIIF, TFIIF alpha

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 10093-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10093-2-AP (GTF2F1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10093-2-AP

  IP Result of anti-GTF2F1 (IP:10093-2-AP, 4ug; Detection:10093-2-AP 1:300) with K-562 cells lysate 4000ug.

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  IHC staining of mouse lung using 10093-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10093-2-AP (GTF2F1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 10093-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10093-2-AP (GTF2F1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat ovary using 10093-2-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 10093-2-AP (GTF2F1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat ovary using 10093-2-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 10093-2-AP (GTF2F1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: Mauslungengewebe, Ratten-Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

10093-2-AP bindet in WB, IP, IHC, ELISA GTF2F1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GTF2F1 fusion protein Ag0133
• Vollständiger Name: general transcription factor IIF, polypeptide 1, 74kDa
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC000120
• Gene symbol: GTF2F1
• Gene ID (NCBI): 2962
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In eukaryotic systems, the initiation of gene transcription involves the ordered assembly of a multiprotein complex on proximal promoter elements, consisting of RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, B, D, E, F and H; and sequence specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit, acting at an intermediate stage in initiation complex formation, binds directly to RNA polymerase II in solution and decrease the affinity of RNA polymerase II for nonspecific DNA. In addition, TFIIF stimulates transcription elongation by RNA polymerase II.

Publikationen

Species	Application	Title
human	WB	Cell Rep; RPAP2 regulates a transcription initiation checkpoint by inhibiting assembly of pre-initiation complex.; Authors - Xinxin Wang
human	WB	Science; Structural visualization of transcription initiation in action; Authors - Xizi Chen