GUCY2D Polyklonaler Antikörper

GUCY2D Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 55127-1-AP

Synonyme

CORD5, CORD6, CYGD, Guanylate cyclase 2D, retinal, GUC1A4, GUC2D, GUCY2D, LCA, LCA1, retGC, RETGC 1, RETGC1, Retinal guanylyl cyclase 1, ROS GC, ROS GC1

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 55127-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55127-1-AP (GUCY2D antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55127-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55127-1-AP (GUCY2D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55127-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55127-1-AP (GUCY2D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

55127-1-AP bindet in WB, IF, ELISA GUCY2D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: guanylate cyclase 2D, membrane (retina-specific)
• Berechnetes Molekulargewicht: 120 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 95 kDa
• GenBank-Zugangsnummer: NM_000180
• Gene symbol: GUCY2D
• Gene ID (NCBI): 3000
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GUCY2D, also named as CORD6, GUC1A4, GUC2D, RETGC, RETGC1 and ROS-GC, belongs to the adenylyl cyclase class-4/guanylyl cyclase family. It probably plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction. A number of nonsense and frameshift mutations in the GUCY2D gene have been identified in LCA1 patients. Defects in GUCY2D are the cause of cone-rod dystrophy type 6 (CORD6). GUCY2D is responsible for many reported cases of autosomal dominant CRDs. The antibody is specific to GUCY2D.

Publikationen

Species	Application	Title
human	IF	iScience; A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation.; Authors - Suguru Yamasaki
mouse	WB,IF	J Biol Chem; The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.; Authors - Poppy Datta