GUF1 Polyklonaler Antikörper

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• KD/KO Validated

GUF1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17489-1-AP

Synonyme

Translation factor GUF1, mitochondrial, Ribosomal back-translocase, GTPase GUF1, Elongation factor 4 homolog, EF-4

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  WB analysis of HepG2 using 17489-1-AP

  WB result of GUF1 antibody (17489-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUF1 transfected HepG2 cells.

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  WB analysis using 17489-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17489-1-AP

  Hela cells were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17489-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 17489-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17489-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17489-1-AP

  IP result of anti-GUF1 (IP:17489-1-AP, 3ug; Detection:17489-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human oesophagus using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human oesophagus using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 17489-1-AP

  Immunofluorescent analysis of HepG2 cells, using GUF1 antibody 17489-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maushirngewebe, Maus-Pankreasgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophagusgewebe, humanes Nierengewebe, humanes Lungenkarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

17489-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA GUF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GUF1 fusion protein Ag11579
• Vollständiger Name: GUF1 GTPase homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 669 aa, 74 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC036768
• Gene symbol: GUF1
• Gene ID (NCBI): 60558
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. In western blotting, we got double bands 74 kDa and 70 kDa, due to the N-terminal 1-49 amino acid sequence that could be cut down.