GYS2 Polyklonaler Antikörper

GYS2 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 22371-1-AP

Synonyme

glycogen synthase 2 (liver), GYS2

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Galerie der Validierungsdaten

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  WB analysis using 22371-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22371-1-AP (GYS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. Mouse heart/ kidney/ skeletal muscle are negative control.

• 

  WB analysis using 22371-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22371-1-AP (GYS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22371-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22371-1-AP (GYS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat liver using 22371-1-AP

  IP result of anti-GYS2 (IP:22371-1-AP, 4ug; Detection:22371-1-AP 1:1000) with rat liver tissue lysate 4000 ug.

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  IHC staining of human liver using 22371-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22371-1-AP (GYS2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 22371-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22371-1-AP (GYS2 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Mausnierengewebe, Rattenlebergewebe
• Erfolgreiche IP: Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 14 publications below
• IHC: See 1 publications below

Produktinformation

22371-1-AP bindet in WB, IP, IHC, ELISA GYS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GYS2 fusion protein Ag18012
• Vollständiger Name: glycogen synthase 2 (liver)
• Berechnetes Molekulargewicht: 703 aa, 81 kDa
• Beobachtetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: BC126310
• Gene symbol: GYS2
• Gene ID (NCBI): 2998
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Nature; Glycogen metabolism links glucose homeostasis to thermogenesis in adipocytes.; Authors - Omer Keinan
rat	WB	Carbohydr Polym; Liver fibrosis alters the molecular structures of hepatic glycogen; Authors - Yujun Wan
mouse	WB	Mol Metab; Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction.; Authors - Ferrol I Rome
rat	WB	Food Funct; l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways; Authors - Ling Lin
rat	WB	Metabolism; Recurrent hypoglycemia increases hepatic gluconeogenesis without affecting glycogen metabolism or systemic lipolysis in rat; Authors - Zejian Liu
rat	WB	Nutrients; Role of Epigallocatechin Gallate in Glucose, Lipid, and Protein Metabolism and L-Theanine in the Metabolism-Regulatory Effects of Epigallocatechin Gallate.; Authors - Ling Lin