Galectin-3 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Galectin-3 Monoklonal Antikörper für IF/ICC, IF-P, FC (Intra)

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, IF-P, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1C1B2

Kat-Nr. : CL594-60207

Synonyme

Gal 3, CBP35, CBP 35, Carbohydrate-binding protein 35, 35 kDa lectin

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  IF Staining of human thyroid cancer using CL594-60207

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using CoraLite®594 Galectin-3 antibody (CL594-60207, Clone: 1C1B2 ) at dilution of 1:200.

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  IF Staining of human thyroid cancer using CL594-60207

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using CoraLite®594 Galectin-3 antibody (CL594-60207, Clone: 1C1B2 ) at dilution of 1:200.

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  IF Staining of human thyroid cancer using CL594-60207

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using CL594-60207 (Galectin-3 antibody) at dilution of 1:50.

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  IF Staining of human thyroid cancer using CL594-60207

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using CL594-60207 (Galectin-3 antibody) at dilution of 1:50.

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  IF Staining of HeLa using CL594-60207

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Galectin-3 antibody (CL594-60207, Clone: 1C1B2 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-60207

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Galectin-3 (CL594-60207, Clone:1C1B2) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Schilddrüsenkarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-60207 bindet in IF/ICC, IF-P, FC (Intra) Galectin-3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Galectin-3 fusion protein Ag6753
• Vollständiger Name: lectin, galactoside-binding, soluble, 3
• Berechnetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC001120
• Gene symbol: Galectin-3
• Gene ID (NCBI): 3958
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).