Giantin Polyklonaler Antikörper

Giantin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Rezensionen (2)

Kat-Nr. : 22270-1-AP

Synonyme

GOLGB1, 372 kDa Golgi complex-associated protein

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• 

  WB analysis using 22270-1-AP

  Various lysates were subjected to Tris-Acetate gel system followed by western blot with 22270-1-AP (Giantin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 22270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22270-1-AP (Giantin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 22270-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Giantin antibody (22270-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 22270-1-AP

  Immunofluorescent analysis of HepG2 cells using 22270-1-AP (Giantin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 22270-1-AP

  Immunofluorescent analysis of HeLa cells using 22270-1-AP (Giantin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 5 publications below

Produktinformation

22270-1-AP bindet in WB, IHC, IF/ICC, ELISA Giantin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: golgin B1, golgi integral membrane protein
• Berechnetes Molekulargewicht: 376 kDa
• Beobachtetes Molekulargewicht: 376 kDa
• GenBank-Zugangsnummer: NM_004487
• Gene symbol: Giantin
• Gene ID (NCBI): 2804
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Giantin is a conserved Golgi membrane protein containing a large cytoplasmic domain. It may function to form the intercisternal cross-bridges of the Golgi complex. Anti-Giantin has been widely used to label the Golgi apparatus.

Publikationen

Species	Application	Title
human	IF	iScience; Cytosolic Ca2+ Modulates Golgi Structure Through PKCα-Mediated GRASP55 Phosphorylation.; Authors - Stephen Ireland
human		Int J Nanomedicine; PMID: 28769563; Authors - Huiying Xue
human	IF	J Alzheimers Dis; High Fat Diet Mediates Amyloid-β Cleaving Enzyme 1 Phosphorylation and SUMOylation, Enhancing Cognitive Impairment in APP/PS1 Mice; Authors - Jian Bao
human	IF	J Biol Chem; Molecular consequences of SCA5 mutations in the spectrin-repeat domains of β-III-spectrin; Authors - Sarah A Denha
human	WB	Methods Mol Biol; Immunofluorescence Microscopy of the Mammalian Golgi Apparatus; Authors - Maryam Arab
monkey	IF	Seizure; Heterozygous RELN missense variants associated with genetic generalized epilepsy; Authors - Xiaoling Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shazia (Verified Customer) (09-24-2025)

A very good giantin antibody with very less background

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence

FH

Boyan (Verified Customer) (04-28-2023)

Detected a band around the expected size, however the signal was really weak.

• Applications: Western Blot