Glucagon Monoklonaler Antikörper

Glucagon Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2A8G1

Kat-Nr. : CL594-67286

Synonyme

GCG, GLP 2, GLP1, GLP2, glucagon, GRPP, GRPP Oxyntomodulin, OXM

---

Galerie der Validierungsdaten

• 

  IF Staining of mouse pancreas using CL594-67286

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using the CoraLite®594-conjugated version of this antibody, CL594-67286 (Glucagon antibody), at dilution of 1:400. The B cells of the islets of Langerhans was labelled in green with CL488-66198(INS antibody).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maus-Pankreasgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67286 bindet in IF Glucagon und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Glucagon fusion protein Ag10629
• Vollständiger Name: glucagon
• Berechnetes Molekulargewicht: 180 aa, 21 kDa
• GenBank-Zugangsnummer: BC005278
• Gene symbol: GCG
• Gene ID (NCBI): 2641
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glucagon is a 29-amino acid peptide hormone secreted from the pancreatic alpha cells with a powerful stimulatory effect on hepatic glucose production acting to increase plasma glucose levels. Glucagon is best known as the counter-regulatory hormone to INS, and normal glucose homeostasis depends largely on the balanced secretion of INS and glucagon from the pancreatic beta and alpha cells, respectively.The regulation of glucose metabolism by glucagon is mediated by its direct action on the peripheral tissues such as the liver and also by the brain. Glucagon is also released postprandially in a transient manner, which was shown to be involved in inhibition of food intake via reduction of meal size.