Glutamine Synthetase Monoklonaler Antikörper

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• KD/KO Validated

Glutamine Synthetase Monoklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(2)

CloneNo.

1B6G4

Kat-Nr. : CL488-66323

Synonyme

GLUL, 1B6G4, EC:2.3.1.225, EC:6.3.1.2, GLNS

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  IF Staining of mouse brain using CL488-66323

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66323 (Glutamine synthetase antibody) at dilution of 1:50.

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  IF Staining of mouse brain using CL488-66323

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Glutamine Synthetase antibody (CL488-66323-2, Clone: 1B6G4 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL488-66323

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Glutamine Synthetase (CL488-66323, Clone:1B6G4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL488-66323 bindet in IF-P, FC (Intra) Glutamine Synthetase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Zebrafisch
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Glutamine Synthetase fusion protein Ag6309
• Vollständiger Name: glutamate-ammonia ligase (glutamine synthetase)
• Berechnetes Molekulargewicht: 374 aa, 42 kDa
• GenBank-Zugangsnummer: BC011700
• Gene symbol: Glutamine Synthetase
• Gene ID (NCBI): 2752
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publikationen

Species	Application	Title
zebrafish	IF	J Cell Sci; Iterative Bleaching Extends Multiplexity (IBEX) facilitates simultaneous identification of all major retinal cell types; Authors - Aanandita A Kothurkar
		Methods Mol Biol; Imaging Cell Signaling in Tissues Using the IBEX Method; Authors - Yuxuan Meng