Glutamine Synthetase Monoklonaler Antikörper

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• KD/KO Validated

Glutamine Synthetase Monoklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(1)

CloneNo.

1B6G4

Kat-Nr. : CL594-66323

Synonyme

GLUL, Glutamate--ammonia ligase, GLNS, EC:6.3.1.2, EC:2.3.1.225

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  IF Staining of mouse brain using CL594-66323

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Glutamine Synthetase antibody (CL594-66323, Clone: 1B6G4 ) at dilution of 1:200.

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  IF Staining of mouse brain using CL594-66323

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Glutamine Synthetase antibody (CL594-66323, Clone: 1B6G4 ) at dilution of 1:200.

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  IF Staining of mouse brain using CL594-66323

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Glutamine Synthetase antibody (CL594-66323-2, Clone: 1B6G4 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL594-66323

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Glutamine Synthetase (CL594-66323, Clone:1B6G4) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL594-66323 bindet in IF-P, FC (Intra) Glutamine Synthetase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Glutamine Synthetase fusion protein Ag6309
• Vollständiger Name: glutamate-ammonia ligase (glutamine synthetase)
• Berechnetes Molekulargewicht: 374 aa, 42 kDa
• GenBank-Zugangsnummer: BC011700
• Gene symbol: Glutamine Synthetase
• Gene ID (NCBI): 2752
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Publikationen

Species	Application	Title
mouse	IF	Pharmacol Res; Ginsenoside Rb1 and berberine synergistically protect against type 2 diabetes mellitus via GDF15/HAMP pathway throughout the liver lobules: Insights from spatial transcriptomics analysis; Authors - Rongfang Guo