GM-CSF Rekombinanter Antikörper

GM-CSF Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

230286E11

Kat-Nr. : 98014-1-RR

Synonyme

Csf2, Gm CSf, Csfgm, CSF 2, Colony Stimulating Factor 2

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  FC experiment of C57BL/6 CD3 T cells using 98014-1-RR

  1x10^6 unstimulated or PMA and ionomycin in presence of protein transport inhibitors stimulated C57BL/6 CD3 T cells were intracellularly stained with 0.25 ug Anti-Mouse Gm-csf (98014-1-RR, Clone: 230286E11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cell were then stained with FITC Plus Anti-Mouse CD3. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98014-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98014-1-RR against Human Gm-csf were performed. The affinity constant is 2.25 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): PMA and ionomycin stimulated C57BL/6 CD3 T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98014-1-RR bindet in FC (Intra) GM-CSF und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: colony stimulating factor 2 (granulocyte-macrophage)
• GenBank-Zugangsnummer: NM-009969
• Gene symbol: GM-CSF
• Gene ID (NCBI): 12981
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Gm-csf, also known as Csf2, is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. Gm-csf was first characterized as a hematopoietic growth factor that stimulates the proliferation of myeloid cells from bone-marrow progenitors. Gm-csf is now recognized as an important activating and differentiation factor for immune cells, and is essential for a wide range of biological processes in both innate and adaptive immunity. Gm-csf has been shown to protect against pulmonary infection and intestinal inflammation, and it is necessary for normal pulmonary and colon homeostasis.