HA Tag Monoklonaler Antikörper

HA Tag Monoklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

rekombinanten Protein und mehr (2)

Anwendung

WB, IF/ICC, IP, CoIP, ChIP, RIP, ELISA, PLA

Konjugation

Unkonjugiert

Publikationen(314)

CloneNo.

1F5C6

Kat-Nr. : 66006-2-Ig

Synonyme

YPYDVPDYA


Filter:
  • Western Blot (WB) analysis of HEK-293 cells using HA Tag Monoclonal antibody (66006-2-Ig)

    WB analysis of HEK-293 using 66006-2-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66006-2-Ig (HA Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ag19172 using HA Tag Monoclonal antibody (66006-2-Ig)

    WB analysis of Ag19172 using 66006-2-Ig

    HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.1μg/mL.

  • Western Blot (WB) analysis of Ag19172 using HA Tag Monoclonal antibody (66006-2-Ig)

    WB analysis of Ag19172 using 66006-2-Ig

    HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.05μg/mL.

  • Western Blot (WB) analysis of Ag19172 using HA Tag Monoclonal antibody (66006-2-Ig)

    WB analysis of Ag19172 using 66006-2-Ig

    HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.025μg/mL.

  • Western Blot (WB) analysis of Ag19172 using HA Tag Monoclonal antibody (66006-2-Ig)

    WB analysis of Ag19172 using 66006-2-Ig

    HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.0125μg/mL.

  • Immunoprecipitation (IP) experiment of Transfected HEK-293 cells using HA Tag Monoclonal antibody (66006-2-Ig)

    IP experiment of Transfected HEK-293 using 66006-2-Ig

    IP result of anti-HA Tag (IP:66006-2-Ig, 5ug; Detection:66006-2-Ig 1:2000) with Transfected HEK-293 cells lysate 1600 ug.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using HA Tag Monoclonal antibody (66006-2-Ig)

    IF Staining of Transfected HEK-293 using 66006-2-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA Tag antibody (66006-2-Ig, Clone: 1F5C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

"HA Tag Antibodies" Comparison

View side-by-side comparison of HA Tag antibodies from other vendors to find the one that best suits your research needs.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBTransfizierte HEK-293-Zellen, HEK-293-Zellen
Erfolgreiche IPTransfizierte HEK-293-Zellen
Erfolgreiche Detektion in IF/ICCTransfizierte HEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:10000-1:100000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunfluoreszenz (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66006-2-Ig bindet in WB, IF/ICC, IP, CoIP, ChIP, RIP, ELISA, PLA HA Tag und zeigt Reaktivität mit rekombinanten Protein

Getestete Reaktivität rekombinanten Protein
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name HA Tag
Gene symbol HA Tag
Gene ID (NCBI) 6
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for HA Tag antibody 66006-2-IgProtokoll herunterladen
IF protocol for HA Tag antibody 66006-2-IgProtokoll herunterladen
IP protocol for HA Tag antibody 66006-2-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
WB

Cell

SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9

Authors - Nora Schmidt
WB,IP

Cell Host Microbe

Microbiota-derived urocanic acid triggered bytyrosine kinase inhibitors potentiates cancer immunotherapy efficacy

Authors - Mengying Zhang

Cell Res

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Authors - Fei Peng
IP

Nat Commun

Targeting the transcription factor HES1 by L-menthol restores protein phosphatase 6 in keratinocytes in models of psoriasis

Authors - Zhikai Wang
IP

J Clin Invest

FAM117B promotes gastric cancer growth and drug resistance by targeting the KEAP1/NRF2 signaling pathway

Authors - Yunjiang Zhou
WB

Nat Microbiol

IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.

Authors - Zhimin Jiang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Parijat (Verified Customer) (03-06-2024)

Good antibody for immunofluorescence. Low background.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: 293T Cells