HA Tag Monoklonaler Antikörper
HA Tag Monoklonal Antikörper für WB, IF/ICC, IP, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
rekombinanten Protein und mehr (2)
Anwendung
WB, IF/ICC, IP, CoIP, ChIP, RIP, ELISA, PLA
Konjugation
Unkonjugiert
CloneNo.
1F5C6
Kat-Nr. : 66006-2-Ig
Synonyme
"HA Tag Antibodies" Comparison
View side-by-side comparison of HA Tag antibodies from other vendors to find the one that best suits your research needs.
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Transfizierte HEK-293-Zellen, HEK-293-Zellen |
Erfolgreiche IP | Transfizierte HEK-293-Zellen |
Erfolgreiche Detektion in IF/ICC | Transfizierte HEK-293-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:10000-1:100000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 226 publications below |
IF | See 28 publications below |
IP | See 68 publications below |
ELISA | See 1 publications below |
CoIP | See 39 publications below |
ChIP | See 3 publications below |
RIP | See 1 publications below |
Produktinformation
66006-2-Ig bindet in WB, IF/ICC, IP, CoIP, ChIP, RIP, ELISA, PLA HA Tag und zeigt Reaktivität mit rekombinanten Protein
Getestete Reaktivität | rekombinanten Protein |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | HA Tag |
Gene symbol | HA Tag |
Gene ID (NCBI) | 6 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-G-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
---|---|
WB protocol for HA Tag antibody 66006-2-Ig | Protokoll herunterladen |
IF protocol for HA Tag antibody 66006-2-Ig | Protokoll herunterladen |
IP protocol for HA Tag antibody 66006-2-Ig | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cell SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9 | ||
Cell Host Microbe Microbiota-derived urocanic acid triggered bytyrosine kinase inhibitors potentiates cancer immunotherapy efficacy | ||
Cell Res Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells. | ||
Nat Commun Targeting the transcription factor HES1 by L-menthol restores protein phosphatase 6 in keratinocytes in models of psoriasis | ||
J Clin Invest FAM117B promotes gastric cancer growth and drug resistance by targeting the KEAP1/NRF2 signaling pathway | ||
Nat Microbiol IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Parijat (Verified Customer) (03-06-2024) | Good antibody for immunofluorescence. Low background.
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