"HA tag Antibodies" Comparison
View side-by-side comparison of HA tag antibodies from other vendors to find the one that best suits your research needs.
|Positive WB detected in||recombinant protein, Transfected HEK-293 cells|
|Positive IP detected in||Transfected HEK-293 cells|
|Positive IF detected in||Transfected HEK-293 cells|
|Western Blot (WB)||WB : 1:5000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
51064-2-AP targets HA tag in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
|Tested Reactivity||recombinant protein|
|Cited Reactivity||human, mouse, rat, chicken, monkey, canine|
|Host / Isotype||Rabbit / IgG|
|Full Name||HA tag|
|Calculated molecular weight||1 kDa|
|Gene ID (NCBI)|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.
Signal Transduct Target Ther
FBXW7β loss-of-function enhances FASN-mediated lipogenesis and promotes colorectal cancer growth
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The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
manohar (Verified Customer) (07-10-2023)
Used Nitrocellulose membrane with 5% milk as blocking for 1 hr, 1% milk used for antibody dilution and kept for overnight incubation
X (Verified Customer) (07-11-2022)
Good for both WB and IF in transfected cells
Victor (Verified Customer) (06-13-2022)
I'm using the antibody for Western blotting tissue culture samples and it works well for my needs for the price. I've been using a 1:2000 dilution which gives me decent bands via Western blot and analyzed using a fluorescent anti-rabbit secondary. The bands could be a little brighter, but this antibody is worth it for the sale price. It's stored in glycerol, so it saves you the trouble of aliquoting and it can be kept at -20 which is nice.
Nick (Verified Customer) (04-20-2022)
The antibody works well and performs consistently in IP and WB
Stephanie (Verified Customer) (01-28-2022)
Antibody easy to use, right recommended dilution. No background observed and clean bands.
S (Verified Customer) (11-22-2021)
Overall, the antibody worked well, but there was a strong band in my control (negative) lane.
K (Verified Customer) (07-15-2021)
This ab recognized the HA tag-ed proteins, but also got a lot of non-specific bands, dilution used were 1:2000
Fen (Verified Customer) (03-06-2020)
it works great for both western blot and IP
Chun (Verified Customer) (12-05-2019)
An excellent antibody
Nikhil (Verified Customer) (10-16-2019)
HA tagged VprBP protein was expressed in H1299 cells. Blue: DAPI; Green:HA-VprBP
Phuong (Verified Customer) (06-19-2019)
HA tag polyclonal antibody works very well. Gives very clean and strong signal.