HA tag Polyclonal antibody

HA tag Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 51064-2-AP

Synonyms

HA tag, YPYDVPDYA



Tested Applications

Positive WB detected inrecombinant protein, Transfected HEK-293 cells
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

51064-2-AP targets HA tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name HA tag
Calculated molecular weight 1 kDa
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.

Protocols

Product Specific Protocols
WB protocol for HA tag antibody 51064-2-APDownload protocol
IF protocol for HA tag antibody 51064-2-APDownload protocol
IP protocol for HA tag antibody 51064-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Bioorg Med Chem Lett

Aegeline, a natural product from the plant Aegle marmelos, mimics the yeast SNARE protein Sec22p in suppressing α-synuclein and Bax toxicity in yeast.

Authors - Asma Derf

Viruses

PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε.

Authors - Fangyi Wu

Biochem Pharmacol

PMID: 30689982

Authors - Min Chen
WB,IF

Nat Neurosci

C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons.

Authors - Rajeeve Sivadasan

MBio

A DNase from a Fungal Phytopathogen Is a Virulence Factor Likely Deployed as Counter Defense against Host-Secreted Extracellular DNA.

Authors - Hee-Jin Park

Dev Cell

FGF15 Activates Hippo Signaling to Suppress Bile Acid Metabolism and Liver Tumorigenesis.

Authors - Suyuan Ji

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Fen (Verified Customer) (03-06-2020)

it works great for both western blot and IP

  • Applications: Western Blot,Immunoprecipitation,
  • Cell Tissue Type: B cells
FH

Chun (Verified Customer) (12-05-2019)

An excellent antibody

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5,000
  • Cell Tissue Type: HEK293 cells
FH

Nikhil (Verified Customer) (10-16-2019)

HA tagged VprBP protein was expressed in H1299 cells. Blue: DAPI; Green:HA-VprBP

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: H1299
HA tag Antibody Immunofluorescence, validation (1:100 dilution) in H1299 (Cat no:51064-2-AP)
FH

Phuong (Verified Customer) (06-19-2019)

HA tag polyclonal antibody works very well. Gives very clean and strong signal.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: 293T