TIM3 Monoklonaler Antikörper

TIM3 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(22)

CloneNo.

4C4G3

Kat-Nr. : 60355-1-Ig

Synonyme

4C4G3, CD366, HAVCR2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3



Geprüfte Anwendungen

Erfolgreiche Detektion in WBDaudi-Zellen, humanes Herzgewebe, PC-3-Zellen, Hausschwein-Thymusgewebe, Rattenherzgewebe, RAW 264.7-Zellen, ROS1728-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

60355-1-Ig bindet in WB, IF, ELISA TIM3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen TIM3 fusion protein Ag16901
Vollständiger Name hepatitis A virus cellular receptor 2
Berechnetes Molekulargewicht 301 aa, 33 kDa
Beobachtetes Molekulargewicht 33 kDa, 50-70 kDa
GenBank-ZugangsnummerBC020843
Gene symbol TIM3
Gene ID (NCBI) 84868
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa detected by this monoclonal antibody probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for TIM3 antibody 60355-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human

EBioMedicine

Immunological profiles of human oligodendrogliomas define two distinct molecular subtypes

Authors - Fan Wu
humanWB

Oncogene

Tumor cell-intrinsic Tim-3 promotes liver cancer via NF-κB/IL-6/STAT3 axis.

Authors - Hualin Zhang
ratWB

Ecotoxicol Environ Saf

Expression levels of key immune indicators and immune checkpoints in manganese-exposed rats

Authors - Yuanmeng Qi
human

J Pathol

Molecular subtyping reveals immune alterations in IDH wild-type lower-grade diffuse glioma.

Authors - Fan Wu
human

Front Cell Dev Biol

Aberrantly Expressed Galectin-9 Is Involved in the Immunopathogenesis of Anti-MDA5-Positive Dermatomyositis-Associated Interstitial Lung Disease.

Authors - Lin Liang
pig

Front Immunol

Up-Regulation of Immune Checkpoints in the Thymus of PRRSV-1-Infected Piglets in a Virulence-Dependent Fashion.

Authors - Inés Ruedas-Torres

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Santhosh (Verified Customer) (04-15-2019)

Human Tonsil FFPE tissue. Heat mediated antigen retrieval (pH 6)

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
TIM3 Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:60355-1-Ig)