Hemoglobin Alpha-Specific Polyklonaler Antikörper

Hemoglobin Alpha-Specific Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19990-1-AP

Synonyme

HBA1, Hemoglobin Alpha, Hemoglobin a, HBA2, Alpha-globin

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  WB analysis of human heart using 19990-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 19990-1-AP (HBA1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 19990-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19990-1-AP (HBA1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 19990-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 19990-1-AP (HBA1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 19990-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 19990-1-AP (Hemoglobin Alpha-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, humanes Herzgewebe, humanes Milzgewebe
• Erfolgreiche Detektion in IHC: human colon; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19990-1-AP bindet in WB, IHC, ELISA Hemoglobin Alpha-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: hemoglobin, alpha 1
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 14-16 kDa
• GenBank-Zugangsnummer: NM_000558
• Gene symbol: HBA1
• Gene ID (NCBI): 3039
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). The antibody is specific to HBA1and has no cross reaction to other globin member.