Hemoglobin Epsilon Polyklonaler Antikörper

Hemoglobin Epsilon Polyklonal Antikörper für WB, IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 12361-1-AP

Synonyme

HBE1, Epsilon globin, Epsilon-globin, HBE, Hemoglobin epsilon chain

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  WB analysis of human placenta using 12361-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 12361-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12361-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12361-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 12361-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 12361-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of human placenta using 12361-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Hemoglobin Epsilon antibody (12361-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 12361-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Hemoglobin Epsilon antibody (12361-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of K-562 using 12361-1-AP

  1x10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human Hemoglobin Epsilon (12361-1-AP)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, K-562-Zellen, Maus-Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in FC (Intra): K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

12361-1-AP bindet in WB, IHC, IF-P, FC (Intra), ELISA Hemoglobin Epsilon und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Hemoglobin Epsilon fusion protein Ag3026
• Vollständiger Name: hemoglobin, epsilon 1
• Berechnetes Molekulargewicht: 147 aa, 16 kDa
• Beobachtetes Molekulargewicht: 12-16 kDa
• GenBank-Zugangsnummer: BC015537
• Gene symbol: HBE1
• Gene ID (NCBI): 3046
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBE1 (hemoglobin epsilon chain) is a beta-type chain of hemoglobin predominantly expressed during the embryonic stage (21321359). The HBE1 has been regarded as the best marker for fetal nucleated red blood cells (NRBCs) . This antibody can be used to label and isolate fetal cells from maternal blood and may be useful in prenatal diagnosis (15906407).

Publikationen

Species	Application	Title
human	WB	Blood; NFI-A directs the fate of hematopoietic progenitors to the erythroid or granulocytic lineage and controls beta-globin and G-CSF receptor expression.; Authors - Linda Marie Starnes
human	WB	Cell Death Differ; Transcriptional fine-tuning of microRNA-223 levels directs lineage choice of human hematopoietic progenitors.; Authors - L Vian
human	WB	Mol Cell Biol; A Feedback Loop Consisting of MicroRNA 23a/27a and the β-Like Globin Suppressors KLF3 and SP1 Regulates Globin Gene Expression.; Authors - Ma Yanni Y
human	WB	Biochim Biophys Acta Mol Cell Res; Long noncoding RNA CCDC26 as a modulator of transcriptional switching between fetal and embryonic globins.; Authors - Tetsuo Hirano
rat	WB	Proteome Sci; A comparison of E15.5 fetus and newborn rat serum proteomes.; Authors - Wei Lilong L
mouse	IHC	Blood; Requirement for antiapoptotic MCL-1 during early erythropoiesis; Authors - Meghan E Turnis