HDAC1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HDAC1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

79

Rezensionen (4)

Kat-Nr. : 10197-1-AP

Synonyme

GON 10, HD1, HDAC1, histone deacetylase 1, RPD3, RPD3L1

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Galerie der Validierungsdaten

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  WB analysis using 10197-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10197-1-AP (HDAC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10197-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10197-1-AP (HDAC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10197-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10197-1-AP (HDAC1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 10197-1-AP

  IP Result of anti-HDAC1 (IP:10197-1-AP, 3ug; Detection:10197-1-AP 1:500) with mouse testis tissue lysate 10000ug.

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  IHC staining of human pancreas cancer using 10197-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10197-1-AP (HDAC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10197-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10197-1-AP (HDAC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10197-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10197-1-AP (HDAC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 10197-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10197-1-AP (HDAC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 10197-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDAC1 antibody (10197-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10197-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDAC1 antibody (10197-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10197-1-AP

  1X10^6 HeLa cells were stained with 0.2ug HDAC1 antibody (10197-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

• 

  FC experiment of SH-SY5Y using 10197-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug HDAC1 antibody (10197-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maushodengewebe, NIH/3T3-Zellen
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:8000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:300-1:1200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 14 publications below
• WB: See 69 publications below
• IHC: See 8 publications below
• IF: See 6 publications below
• IP: See 8 publications below
• CoIP: See 5 publications below
• ChIP: See 3 publications below

Produktinformation

10197-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA HDAC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HDAC1 fusion protein Ag0256
• Vollständiger Name: histone deacetylase 1
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC000301
• Gene symbol: HDAC1
• Gene ID (NCBI): 3065
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HDAC1 poly https://www.ptglab.com/products/HDAC1-Antibody-10197-1-AP.htm

Background

Histone Deacetylase 1 (HDAC1) is a nuclear localized class I histone deacetylase that plays a role in the regulation of gene expression, mainly by repression of gene activity. Additionally, HDAC can mediate deacetylation of a subset of non-histone proteins, leading to their degradation. HDAC1 activity has an impact on cell growth, proliferation, and death.

1. What is the molecular weight of HDAC1?

The molecular size of HDAC1 is 60 kDa.

2. What is the subcellular localization of HDAC1?

Unlike some HDACs, HDAC1 is present entirely in the nucleus. Our HDAC1 antibody has been broadly tested for IF/ICC.

3. I cannot detect an HDAC1 specific signal in my sample during western blotting.

Make sure that you efficiently extract nuclear proteins during your sample preparation. Some lysis buffers (e.g., based on Triton X-100) may not extract nuclear proteins. We recommend using RIPA buffer (https://www.ptglab.com/support/protocols/). We highly recommend using nuclear loading control antibodies, such as lamin B1 or PCNA (https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/). Alternatively, you may consider performing cell fractionation.

4. How do I perform chromatin immunoprecipitation (ChiP) with the HDAC1 antibody?

We recommend using our standard chromatin immunoprecipitation protocol (https://www.ptglab.com/media/2708/web_chip-protocol.pdf).

5. Is HDAC1 post-translationally modified?

HDAC1 is a protein deacetylase but itself can also be a subject of post-translational modifications, including phosphorylation, acetylation, ubiquitination, SUMOylation, nitrosylation, and carbonylation (PMID: 21197454).

6. What is the role of HDAC1 in cancer?

The acetylation and deacetylation of histones play an important part in the epigenetic regulation of gene expression. Alterations in the balance between these two opposing processes changes chromosome remodeling and increased deacetylation leads to gene silencing. HDAC1 levels are increased in many cancer cell types and the role of HDAC1 in cancer is connected not only to histone deacetylation but also to the deacetylation of other proteins, such as Rb family proteins, estrogen receptors, and p53 protein (PMID: 19383284).

Publikationen

Species	Application	Title
human	WB	Acta Pharm Sin B; Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.; Authors - Xin Sun
mouse	WB	J Clin Invest; Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.; Authors - Shanshan Zhong
human	WB	Nat Commun; PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression.; Authors - Xianchun Lan
mouse	IP	Nat Commun; The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression.; Authors - Xinzhi Li
human	WB,IHC	Hepatology; PROX1 promotes hepatocellular carcinoma metastasis by way of up-regulating hypoxia-inducible factor 1α expression and protein stability.; Authors - Liu Yanfeng Y
mouse	WB,IF,IP	J Exp Med; ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.; Authors - Longfei Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mi (Verified Customer) (06-25-2023)

Works pretty well in human adipocyte cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human white adipocytes

FH

Christos (Verified Customer) (04-03-2023)

Separating Gel: 10% poly-acrylamide Transfer at 400mA for 2hr onto nitrocellulose membrane at 4oC Blocking and antibodies dilutions: 5% non-fat milk in PBS-Tween20 for 1hr Incubation with primary Abs: overnight at 4oC Incubation with secondary Abs: 1hr at 4oC

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human cervix carcinoma cells/ HeLe Kyoto

HDAC1 Antibody Western Blot validation (1/1000 dilution) in Human cervix carcinoma cells/ HeLe Kyoto (Cat no:10197-1-AP)

FH

Dipen (Verified Customer) (07-20-2020)

Great antibody for Western blotting.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hippocampal neurons

HDAC1 Antibody Western Blot, validation (1:1000 dilution) in Hippocampal neurons (Cat no:10197-1-AP)

FH

Uxoa (Verified Customer) (02-19-2020)

HDAC1 in human primary fibroblasts untreated vs treated. Cells fixed with 4% PFA 15 min RT. HDAC1 incubation 1:50 in PBST (0.1% triton) + 10% DS O/N 4ºC. Donkey anti-rabbit Alexa fluor 555 1:500 1h RT.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Human fibroblasts

HDAC1 Antibody Immunofluorescence, validation (1:50 dilution) in Human fibroblasts (Cat no:10197-1-AP)

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