HDAC6 Monoklonaler Antikörper

HDAC6 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1C7C3

Kat-Nr. : CL594-67250

Synonyme

FLJ16239, HD6, HDAC6, histone deacetylase 6, JM21, KIAA0901



Geprüfte Anwendungen

Erfolgreiche Detektion in IFSH-SY5Y-Zellen
Erfolgreiche Detektion in FCSH-SY5Y-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67250 bindet in IF, FC (Intra) HDAC6 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen HDAC6 fusion protein Ag28585
Vollständiger Name histone deacetylase 6
Berechnetes Molekulargewicht 1063 aa, 114 kDa, 131 kDa
Beobachtetes Molekulargewicht 150-160 kDa
GenBank-ZugangsnummerBC013737
Gene symbol HDAC6
Gene ID (NCBI) 10013
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. HDAC6 has some splicing variants such as P114(~130kd), P131(~160kd).This antibody is raised against residues near the C terminal of human HDAC6. The calculated molecular weight of HDAC6 is 130 kDa, but the modified the HDAC6 is about 150-160 kDa.

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 HDAC6 antibody CL594-67250Protokoll herunterladen
FC protocol for CL594 HDAC6 antibody CL594-67250Protokoll herunterladen
Standard-Protokolle
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