HDGF Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HDGF Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Kat-Nr. : 11344-1-AP

Synonyme

HDGF, Hepatoma derived growth factor, HMG 1L2, HMG1L2

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Galerie der Validierungsdaten

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  WB analysis using 11344-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11344-1-AP (HDGF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11344-1-AP

  IP Result of anti-HDGF (IP:11344-1-AP, 3ug; Detection:11344-1-AP 1:1000) with HepG2 cells lysate 3000ug.

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  IHC staining of human lung cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 11344-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11344-1-AP (HDGF antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HEK-293 using 11344-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using HDGF antibody (11344-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293T-Zellen, HeLa-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 8 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

11344-1-AP bindet in WB, IP, IHC, IF, CoIP, ChIP, ELISA HDGF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HDGF fusion protein Ag1893
• Vollständiger Name: hepatoma-derived growth factor (high-mobility group protein 1-like)
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC018991
• Gene symbol: HDGF
• Gene ID (NCBI): 3068
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HDGF, also named as HMG1L2, belongs to the HDGF family. HDGF is a mitogen for many cell types with nuclear localization necessary for its mitogenic activity. HDGF has also been identified as an important prognostic marker in pathologic cell growth, as it is overexpressed in a number of cancers with expression linked to a poor outcome in lung, esophageal, pancreatic and hepatic cancer.HDGF is highly expressed in human NSCLC tissues, predicting worse prognosis in resected NSCLCs. It might be useful molecular biomarker for predicting the prognosis of resected NSCLCs.

Publikationen

Species	Application	Title
human	WB	J Hazard Mater; Proteomic analysis of cell proliferation in a human hepatic cell line (HL-7702) induced by perfluorooctane sulfonate using iTRAQ.; Authors - Ruina Cui
human	WB,IHC,IF,ChIP	Mol Oncol; Role of hepatoma-derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma.; Authors - Xuejie Min ; KD Validated
human	WB	Front Cell Dev Biol; NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma.; Authors - Ye-Wei Zhang
human	WB	Eur J Cancer; Identification of glia maturation factor beta as an independent prognostic predictor for serous ovarian cancer.; Authors - Li Yan Li YL
human	WB	Hum Cell; LncRNA SNHG3 promotes cell proliferation and invasion through the miR-384/hepatoma-derived growth factor axis in breast cancer.; Authors - Qiuhong Ma ; KD Validated
human	WB,IF,IP	Cancer Cell Int; NAP1L1 interacts with hepatoma-derived growth factor to recruit c-Jun inducing breast cancer growth.; Authors - Shu Liu