HE4 Monoklonaler Antikörper

HE4 Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

5B2D9

Kat-Nr. : CL594-66557

Synonyme

WFDC2, WAP5, WAP four-disulfide core domain protein 2, WAP four disulfide core domain protein 2, Major epididymis-specific protein E4

---

• 

  IF Staining of SKOV-3 using CL594-66557

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using CoraLite®594 HE4 antibody (CL594-66557, Clone: 5B2D9 ) at dilution of 1:200.

• 

  IF Staining of SKOV-3 using CL594-66557

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite®®594 HE4 antibody (CL594-66557, Clone: 5B2D9 ) at dilution of 1:200.

• 

  FC experiment of OVCAR-3 using CL594-66557

  1x10^6 OVCAR-3 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated HE4 Monoclonal antibody (CL594-66557, Clone:5B2D9)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (1F8D3) (CL594-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of SKOV-3 using CL594-66557

  1x10^6 SKOV-3 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated HE4 Monoclonal antibody (CL594-66557, Clone:5B2D9)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: SKOV-3-Zellen
• Erfolgreiche Detektion in FC (Intra): SKOV-3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66557 bindet in IF/ICC HE4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HE4 fusion protein Ag5936
• Vollständiger Name: WAP four-disulfide core domain 2
• Berechnetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC046106
• Gene symbol: HE4
• Gene ID (NCBI): 10406
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.